› 5k2h

Electron crystallography
1.05Å resolution

Structure of GNNQQNY from yeast prion Sup35 in space group P212121 determined by MicroED

Released:
DOI: 10.2210/pdb5k2h/pdb
PDB entry 5k2h coloured by chain, front view.
PDB entry 5k2h coloured by chain, side view.
PDB entry 5k2h coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Ab initio structure determination from prion nanocrystals at atomic resolution by MicroED.
Sawaya MR, Rodriguez J, Cascio D, Collazo MJ, Shi D, Reyes FE, Hattne J, Gonen T, Eisenberg DS
Proc Natl Acad Sci U S A 113 11232-11236 (2016)
PMID: 27647903
Related structures: EMD-8199

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo octadecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138676 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Eukaryotic peptide chain release factor GTP-binding subunit Chain: A
Molecule details ›
Chain: A
Length: 7 amino acids
Theoretical weight: 837 Da
Source organism: Saccharomyces cerevisiae
Expression system: Not provided
UniProt:
  • Canonical: P05453 (Residues: 7-13; Coverage: 1%)
Gene names: GST1, PNM2, SAL3, SUF12, SUP2, SUP35, YD9395.05, YDR172W

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 1.05Å
Relevant EMDB volumes: EMD-8199
Expression system: Not provided

Quick links

Citations

15 review citations

A new era for understanding amyloid structures and disease.
Iadanza et al. (2018)
14 more

5 mentions without citation

MicroED opens a new era for biological structure determination.
Nannenga et al. (2016)
4 more