5k66

X-ray diffraction
2Å resolution

Crystal structure of N-terminal amidase with Asn-Glu peptide

Released:
Model geometry
Fit model/data
Primary publication:
Structural basis for dual specificity of yeast N-terminal amidase in the N-end rule pathway.
Proc Natl Acad Sci U S A 113 12438-12443 (2016)
PMID: 27791147

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-126099 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CN hydrolase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 459 amino acids
Theoretical weight: 52.05 KDa
Source organism: Saccharomyces cerevisiae CEN.PK113-7D
Expression system: Escherichia coli
UniProt:
  • Canonical: N1P8Q8 (Residues: 1-457; Coverage: 100%)
Gene name: CENPK1137D_1355
Sequence domains: Carbon-nitrogen hydrolase

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: PAL/PLS BEAMLINE 5C (4A)
Spacegroup: I4122
Unit cell:
a: 134.602Å b: 134.602Å c: 119.144Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.177 0.175 0.215
Expression system: Escherichia coli