› 5kcc

X-ray diffraction
2.39Å resolution

Crystal Structure of the ER-alpha Ligand-binding Domain (Y537S) in Complex with Oxabicyclic Heptene Sulfonamide (OBHS-N)

Released:
DOI: 10.2210/pdb5kcc/pdb
PDB entry 5kcc coloured by chain, front view.
PDB entry 5kcc coloured by chain, side view.
PDB entry 5kcc coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Full antagonism of the estrogen receptor without a prototypical ligand side chain.
Srinivasan S, Nwachukwu JC, Bruno NE, Dharmarajan V, Goswami D, Kastrati I, Novick S, Nowak J, Cavett V, Zhou HB, Boonmuen N, Zhao Y, Min J, Frasor J, Katzenellenbogen BS, Griffin PR, Katzenellenbogen JA, Nettles KW
OpenAccess logo Nat Chem Biol 13 111-118 (2017)
PMID: 27870835

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136928 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Estrogen receptor Chains: A, B
Molecule details ›
Chains: A, B
Length: 257 amino acids
Theoretical weight: 29.3 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P03372 (Residues: 298-554; Coverage: 43%)
Gene names: ESR, ESR1, NR3A1
Sequence domains: Ligand-binding domain of nuclear hormone receptor
Structure domains: Retinoid X Receptor
Nuclear receptor coactivator 2 Chains: C, D
Molecule details ›
Chains: C, D
Length: 14 amino acids
Theoretical weight: 1.67 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q15596 (Residues: 686-699; Coverage: 1%)
Gene names: BHLHE75, NCOA2, SRC2, TIF2

Ligands and Environments

1 bound ligand:
Ligand OB1 2 x OB1
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P21
Unit cell:
a: 54.53Å b: 81.16Å c: 58.68Å
α: 90° β: 110.8° γ: 90°
R-values:
R R work R free
0.228 0.224 0.265
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

4 review citations

Structural underpinnings of oestrogen receptor mutations in endocrine therapy resistance.
Katzenellenbogen et al. (2018)
3 more

1 mention without citation

Full antagonism of the estrogen receptor without a prototypical ligand side chain.
Srinivasan et al. (2017)