› 5ker

X-ray diffraction
2.2Å resolution

Deer mouse recombinant hemoglobin from high altitude species

Released:
DOI: 10.2210/pdb5ker/pdb
PDB entry 5ker coloured by chain, front view.
PDB entry 5ker coloured by chain, side view.
PDB entry 5ker coloured by chain, top view.
Source organism: Peromyscus maniculatus
Primary publication:
Alteration of the α1β2/α2β1 subunit interface contributes to the increased hemoglobin-oxygen affinity of high-altitude deer mice.
Inoguchi N, Mizuno N, Baba S, Kumasaka T, Natarajan C, Storz JF, Moriyama H
OpenAccess logo PLoS One 12 e0174921 (2017)
PMID: 28362841

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107528 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Globin domain-containing protein Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 141 amino acids
Theoretical weight: 14.89 KDa
Source organism: Peromyscus maniculatus
Expression system: Escherichia coli
UniProt:
  • Canonical: A4ZQ87 (Residues: 2-142; Coverage: 99%)
Gene names: HBA, HBAT1, Hba
Sequence domains: Globin
Structure domains: Globins
Globin domain-containing protein Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 146 amino acids
Theoretical weight: 15.79 KDa
Source organism: Peromyscus maniculatus
Expression system: Escherichia coli
UniProt:
  • Canonical: B7SBL4 (Residues: 2-147; Coverage: 99%)
Gene names: HBB, HBB-T1
Sequence domains: Globin
Structure domains: Globins

Ligands and Environments

1 bound ligand:
Ligand HEM 8 x HEM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P21
Unit cell:
a: 53.182Å b: 229.618Å c: 53.277Å
α: 90° β: 119.12° γ: 90°
R-values:
R R work R free
0.194 0.193 0.202
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

Acclimatization of low altitude-bred deer mice ( Peromyscus maniculatus) to high altitude.
Dane et al. (2018)

2 mentions without citation

Alteration of the α1β2/α2β1 subunit interface contributes to the increased hemoglobin-oxygen affinity of high-altitude deer mice.
Inoguchi et al. (2017)
1 more