› 5khl

X-ray diffraction
2.4Å resolution

Crystal Structure of periplasmic Heme binding protein HutB of Vibrio cholerae

Released:
DOI: 10.2210/pdb5khl/pdb
PDB entry 5khl coloured by chain, front view.
PDB entry 5khl coloured by chain, side view.
PDB entry 5khl coloured by chain, top view.
Source organism: Vibrio cholerae O395
Entry authors: Agarwal S, Dey S, Ghosh B, Dasgupta J

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101760 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fe/B12 periplasmic-binding domain-containing protein Chain: B
Molecule details ›
Chain: B
Length: 271 amino acids
Theoretical weight: 28.86 KDa
Source organism: Vibrio cholerae O395
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0H3AE70 (Residues: 24-277; Coverage: 99%)
Gene names: VC0395_0324, hutB
Sequence domains: Periplasmic binding protein
Structure domains: Nitrogenase molybdenum iron protein domain

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RRCAT INDUS-2 BEAMLINE PX-BL21
Spacegroup: P43212
Unit cell:
a: 62.884Å b: 62.884Å c: 135.774Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.2 0.256
Expression system: Escherichia coli BL21(DE3)

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