Function and Biology

MutY N-terminal domain in complex with undamaged DNA

Source organism: Geobacillus stearothermophilus
Biochemical function: 4 iron, 4 sulfur cluster binding
Biological process: base-excision repair
Cellular component: not assigned

EC 3.2.2.31: Adenine glycosylase

Reaction catalysed:
Hydrolyzes free adenine bases from 7,8-dihydro-8-oxoguanine:adenine mismatched double-stranded DNA, leaving an apurinic site.
Systematic name:
Adenine-DNA deoxyribohydrolase (adenine-releasing)
Alternative Name(s):
  • A/G-specific adenine glycosylase
  • MutY (gene name)

Sequence families

Pfam Protein families (Pfam)
PF10576
Domain description: Iron-sulfur binding domain of endonuclease III
Occurring in:
  1. Adenine DNA glycosylase
The deposited structure of PDB entry 5kn8 contains 1 copy of Pfam domain PF10576 (Iron-sulfur binding domain of endonuclease III) in Adenine DNA glycosylase. Showing 1 copy in chain A.

PF00633
Domain description: Helix-hairpin-helix motif
Occurring in:
  1. Adenine DNA glycosylase
The deposited structure of PDB entry 5kn8 contains 1 copy of Pfam domain PF00633 (Helix-hairpin-helix motif) in Adenine DNA glycosylase. Showing 1 copy in chain A.

PF00730
Domain description: HhH-GPD superfamily base excision DNA repair protein
Occurring in:
  1. Adenine DNA glycosylase
The deposited structure of PDB entry 5kn8 contains 1 copy of Pfam domain PF00730 (HhH-GPD superfamily base excision DNA repair protein) in Adenine DNA glycosylase. Showing 1 copy in chain A.

InterPro InterPro annotations
IPR003265
Domain description: HhH-GPD domain
Occurring in:
  1. Adenine DNA glycosylase
IPR003651
Domain description: Endonuclease III-like, iron-sulphur cluster loop motif
Occurring in:
  1. Adenine DNA glycosylase
IPR000445
Domain description: Helix-hairpin-helix motif
Occurring in:
  1. Adenine DNA glycosylase
IPR023170
Domain description: Helix-hairpin-helix, base-excision DNA repair, C-terminal
Occurring in:
  1. Adenine DNA glycosylase
IPR005760
Domain description: A/G-specific adenine glycosylase MutY
Occurring in:
  1. Adenine DNA glycosylase
IPR011257
Domain description: DNA glycosylase
Occurring in:
  1. Adenine DNA glycosylase
IPR044298
Domain description: Adenine/Thymine-DNA glycosylase
Occurring in:
  1. Adenine DNA glycosylase

Structure domains

CATH CATH domains
1.10.1670.10
Class: Mainly Alpha
Architecture: Orthogonal Bundle
Topology: Endonuclease Iii, domain 2
Homology: Helix-hairpin-Helix base-excision DNA repair enzymes (C-terminal)
Occurring in:
  1. Adenine DNA glycosylase
The deposited structure of PDB entry 5kn8 contains 1 copy of CATH domain 1.10.1670.10 (Endonuclease Iii, domain 2) in Adenine DNA glycosylase. Showing 1 copy in chain A.
1.10.340.30
Class: Mainly Alpha
Architecture: Orthogonal Bundle
Topology: Endonuclease III; domain 1
Homology: Hypothetical protein; domain 2
Occurring in:
  1. Adenine DNA glycosylase
The deposited structure of PDB entry 5kn8 contains 1 copy of CATH domain 1.10.340.30 (Endonuclease III; domain 1) in Adenine DNA glycosylase. Showing 1 copy in chain A.