› 5knq

X-ray diffraction
2.55Å resolution

Crystal structure of Mycobacterium tuberculosis hypoxanthine guanine phosphoribosyltransferase in complex with [3S,4R]-(4-(Guanin-9-yl)pyrrolidin-3-yl)oxymethanephosphonic acid and pyrophosphate

Released:
DOI: 10.2210/pdb5knq/pdb
PDB entry 5knq coloured by chain, front view.
PDB entry 5knq coloured by chain, side view.
PDB entry 5knq coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Entry authors: Eng WS, Rejman D, Keough DT, Guddat LW

Function and Biology Details

Reaction catalysed: 
IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-551998 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hypoxanthine-guanine phosphoribosyltransferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 207 amino acids
Theoretical weight: 22.97 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WHQ9 (Residues: 2-202; Coverage: 100%)
Gene names: MTCY15C10.28, Rv3624c, hprT, hpt
Sequence domains: Phosphoribosyl transferase domain
Structure domains: Rossmann fold

Ligands and Environments

3 bound ligands:
Ligand 6W8 4 x 6W8
Ligand MG 8 x MG
Ligand POP 4 x POP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Spacegroup: P21
Unit cell:
a: 56.262Å b: 85.507Å c: 81.468Å
α: 90° β: 107.22° γ: 90°
R-values:
R R work R free
0.191 0.187 0.237
Expression system: Escherichia coli

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