› 5knr

X-ray diffraction
2.86Å resolution

E. coli HPRT in complexed with 9-[(N-phosphonoethyl-N-phosphonoethoxyethyl)-2-aminoethyl]-guanine

Released:
DOI: 10.2210/pdb5knr/pdb
PDB entry 5knr coloured by chain, front view.
PDB entry 5knr coloured by chain, side view.
PDB entry 5knr coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal Structures of Acyclic Nucleoside Phosphonates in Complex with Escherichia coli Hypoxanthine Phosphoribosyltransferase
Eng WS, Hockova D, Spacek P, Baszczynski O, Janeba Z, Naesens L, Keough DT, Guddat LW
Chemistryselect 1 6267-6276 (2016)

Function and Biology Details

Reaction catalysed: 
IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-532393 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hypoxanthine phosphoribosyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 182 amino acids
Theoretical weight: 20.64 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A9M2 (Residues: 1-178; Coverage: 100%)
Gene names: JW5009, b0125, hpt
Sequence domains: Phosphoribosyl transferase domain
Structure domains: Rossmann fold

Ligands and Environments

2 bound ligands:
Ligand 3L5 2 x 3L5
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Spacegroup: P3121
Unit cell:
a: 84.617Å b: 84.617Å c: 167.116Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.236 0.233 0.267
Expression system: Escherichia coli

Quick links