› 5kth

X-ray diffraction
2.21Å resolution

Structure of cow mincle complexed with brartemicin

Released:
DOI: 10.2210/pdb5kth/pdb
PDB entry 5kth coloured by chain, front view.
PDB entry 5kth coloured by chain, side view.
PDB entry 5kth coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Binding Sites for Acylated Trehalose Analogs of Glycolipid Ligands on an Extended Carbohydrate Recognition Domain of the Macrophage Receptor Mincle.
Feinberg H, Rambaruth ND, Jégouzo SA, Jacobsen KM, Djurhuus R, Poulsen TB, Weis WI, Taylor ME, Drickamer K
OpenAccess logo J Biol Chem 291 21222-21233 (2016)
PMID: 27542410

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-122748 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
C-type lectin domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 149 amino acids
Theoretical weight: 17.3 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: E1BHM0 (Residues: 64-208; Coverage: 69%)
Gene name: CLEC4E
Sequence domains: Lectin C-type domain
Structure domains: Mannose-Binding Protein A, subunit A

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
3 bound ligands:
Ligand 6X7 2 x 6X7
Ligand CA 3 x CA
Ligand PGE 1 x PGE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P3121
Unit cell:
a: 98.332Å b: 98.332Å c: 45.636Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.17 0.168 0.21
Expression system: Escherichia coli

Quick links

Citations

14 review citations

Contact, Collaboration, and Conflict: Signal Integration of Syk-Coupled C-Type Lectin Receptors.
Ostrop et al. (2017)
13 more

1 mention without citation

Binding Sites for Acylated Trehalose Analogs of Glycolipid Ligands on an Extended Carbohydrate Recognition Domain of the Macrophage Receptor Mincle.
Feinberg et al. (2016)