› 5l00

X-ray diffraction
1.25Å resolution

Self-complimentary RNA 15mer binding with GMP monomers

Released:
DOI: 10.2210/pdb5l00/pdb
PDB entry 5l00 coloured by chain, front view.
PDB entry 5l00 coloured by chain, side view.
PDB entry 5l00 coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
Unusual Base-Pairing Interactions in Monomer-Template Complexes.
Zhang W, Tam CP, Wang J, Szostak JW
OpenAccess logo ACS Cent Sci 2 916-926 (2016)
PMID: 28058281

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-196945 (preferred)
Entry contents:
1 distinct RNA molecule
Macromolecule:
RNA (5'-R(*(LCC)P*(LCC)P*(LCC)P*(LCG)P*AP*CP*UP*UP*AP*AP*GP*UP*CP*GP*G)-3') Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 15 nucleotides
Theoretical weight: 4.86 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand G 4 x G
Ligand MG 4 x MG
1 modified residue:
Ligand LCG 4 x LCG

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P3
Unit cell:
a: 43.846Å b: 43.846Å c: 86.45Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.21 0.209 0.239
Expression system: Not provided

Quick links

Citations

1 review citation

The Narrow Road to the Deep Past: In Search of the Chemistry of the Origin of Life.
Szostak JW. (2017)
15 other articles

2 mentions without citation

Unusual Base-Pairing Interactions in Monomer-Template Complexes.
Zhang et al. (2016)
1 more