› 5l7f

X-ray diffraction
1.8Å resolution

Crystal structure of MMP12 mutant K421A in complex with RXP470.1 conjugated with fluorophore Cy5,5 in space group P21.

Released:
DOI: 10.2210/pdb5l7f/pdb
PDB entry 5l7f coloured by chain, front view.
PDB entry 5l7f coloured by chain, side view.
PDB entry 5l7f coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Synthesis and in Vitro and in Vivo Evaluation of MMP-12 Selective Optical Probes.
Bordenave T, Helle M, Beau F, Georgiadis D, Tepshi L, Bernes M, Ye Y, Levenez L, Poquet E, Nozach H, Razavian M, Toczek J, Stura EA, Dive V, Sadeghi MM, Devel L
Bioconjug Chem 27 2407-2417 (2016)
PMID: 27564088

Function and Biology Details

Reaction catalysed: 
Hydrolysis of soluble and insoluble elastin (1). Specific cleavages are also produced at -Ala(14)-|-Leu- and -Tyr(16)-|-Leu- in the B chain of insulin (2).
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153971 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Macrophage metalloelastase Chains: A, B
Molecule details ›
Chains: A, B
Length: 159 amino acids
Theoretical weight: 17.56 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P39900 (Residues: 106-263; Coverage: 35%)
Gene names: HME, MMP12
Sequence domains: Matrixin
Structure domains: Collagenase (Catalytic Domain)

Ligands and Environments

8 bound ligands:
Ligand ZN 4 x ZN
Ligand CA 6 x CA
Ligand BR 1 x BR
Ligand EDO 3 x EDO
Ligand R47 2 x R47
Ligand 6PJ 2 x 6PJ
Ligand GOL 1 x GOL
Ligand DMS 1 x DMS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P21
Unit cell:
a: 48.92Å b: 70.37Å c: 52.22Å
α: 90° β: 104.61° γ: 90°
R-values:
R R work R free
0.177 0.176 0.195
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Optical Molecular Imaging of Inflammatory Cells in Interventional Medicine-An Emerging Strategy.
Birch et al. (2019)
1 more

1 mention without citation

Synthesis and in Vitro and in Vivo Evaluation of MMP-12 Selective Optical Probes.
Bordenave et al. (2016)