› 5l94

X-ray diffraction
2.25Å resolution

The 2.25 A crystal structure of CYP109E1 from Bacillus megaterium in complex with testosterone

Released:
DOI: 10.2210/pdb5l94/pdb
Model geometry
Fit model/data
PDB entry 5l94 coloured by chain, front view.
PDB entry 5l94 coloured by chain, side view.
PDB entry 5l94 coloured by chain, top view.
Source organism: Priestia megaterium DSM 319
Primary publication:
Structural basis of steroid binding and oxidation by the cytochrome P450 CYP109E1 from Bacillus megaterium.
Jóźwik IK, Kiss FM, Gricman Ł, Abdulmughni A, Brill E, Zapp J, Pleiss J, Bernhardt R, Thunnissen AW
OpenAccess logo FEBS J 283 4128-4148 (2016)
PMID: 27686671

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112687 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome P450 Chains: A, B
Molecule details ›
Chains: A, B
Length: 410 amino acids
Theoretical weight: 47.82 KDa
Source organism: Priestia megaterium DSM 319
Expression system: Escherichia coli
UniProt:
  • Canonical: D5DKI8 (Residues: 1-404; Coverage: 100%)
Gene name: BMD_3874
Sequence domains: Cytochrome P450
Structure domains: Cytochrome P450

Ligands and Environments

2 bound ligands:
Ligand HEM 2 x HEM
Ligand TES 1 x TES
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: BRUKER AXS MICROSTAR
Spacegroup: P21
Unit cell:
a: 60.359Å b: 134.922Å c: 61.87Å
α: 90° β: 113.83° γ: 90°
R-values:
R R work R free
0.221 0.219 0.263
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Bacterial steroid hydroxylases: enzyme classes, their functions and comparison of their catalytic mechanisms.
Szaleniec et al. (2018)
3 more

2 mentions without citation

Structural basis of steroid binding and oxidation by the cytochrome P450 CYP109E1 from Bacillus megaterium.
Jóźwik et al. (2016)
1 more