› 5lb3

X-ray diffraction
1.8Å resolution

Crystal structure of human RECQL5 helicase in complex with ADP/Mg.

Released:
DOI: 10.2210/pdb5lb3/pdb
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PDB entry 5lb3 coloured by chain, front view.
PDB entry 5lb3 coloured by chain, side view.
PDB entry 5lb3 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Insights into the RecQ helicase mechanism revealed by the structure of the helicase domain of human RECQL5.
Newman JA, Aitkenhead H, Savitsky P, Gileadi O
OpenAccess logo Nucleic Acids Res 45 4231-4243 (2017)
PMID: 28100692

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131739 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ATP-dependent DNA helicase Q5 Chains: B, E
Molecule details ›
Chains: B, E
Length: 445 amino acids
Theoretical weight: 49.54 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O94762 (Residues: 11-453; Coverage: 45%)
Gene names: RECQ5, RECQL5
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand ZN 2 x ZN
Ligand ADP 2 x ADP
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P21
Unit cell:
a: 72.757Å b: 62.943Å c: 104.703Å
α: 90° β: 95.44° γ: 90°
R-values:
R R work R free
0.197 0.195 0.234
Expression system: Escherichia coli BL21(DE3)

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Citations

6 review citations

History of DNA Helicases.
Brosh et al. (2020)
5 more

4 mentions without citation

New Insights Into DNA Helicases as Druggable Targets for Cancer Therapy.
Datta et al. (2018)
3 more

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