› 5lc6

X-ray diffraction
1.7Å resolution

Crystal structure of a single chain monellin mutant: Q28K/C41S/Y65R-MNEI

Released:
DOI: 10.2210/pdb5lc6/pdb
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PDB entry 5lc6 coloured by chain, front view.
PDB entry 5lc6 coloured by chain, side view.
PDB entry 5lc6 coloured by chain, top view.
Source organism: Dioscoreophyllum cumminsii
Primary publication:
Sweeter and stronger: enhancing sweetness and stability of the single chain monellin MNEI through molecular design.
Leone S, Pica A, Merlino A, Sannino F, Temussi PA, Picone D
OpenAccess logo Sci Rep 6 34045 (2016)
PMID: 27658853

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136325 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Monellin chain A; Monellin chain B Chains: A, B
Molecule details ›
Chains: A, B
Length: 96 amino acids
Theoretical weight: 11.27 KDa
Source organism: Dioscoreophyllum cumminsii
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P02882 (Residues: 1-48; Coverage: 96%)
  • Canonical: P02881 (Residues: 1-45; Coverage: 100%)
Sequence domains: Monellin
Structure domains: Nuclear Transport Factor 2; Chain: A,

Ligands and Environments

1 bound ligand:
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ELETTRA BEAMLINE 5.2R
Spacegroup: C2
Unit cell:
a: 129.3Å b: 30.99Å c: 45.5Å
α: 90° β: 106.17° γ: 90°
R-values:
R R work R free
0.191 0.188 0.229
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

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Citations

3 review citations

Sweeteners and sweetness enhancers.
Belloir et al. (2017)
2 more

2 mentions without citation

Sweeter and stronger: enhancing sweetness and stability of the single chain monellin MNEI through molecular design.
Leone et al. (2016)
1 more

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