5ldp

X-ray diffraction
1.8Å resolution

Crystal structure of E.coli LigT complexed with ATP

Released:

Function and Biology Details

Reaction catalysed:
(Ribonucleotide)(n)-2',3'-cyclic phosphate + H(2)O = (ribonucleotide)(n)-2'-phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-102949 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA 2',3'-cyclic phosphodiesterase Chains: A, B
Molecule details ›
Chains: A, B
Length: 177 amino acids
Theoretical weight: 20.05 KDa
Source organism: Escherichia coli BL21(DE3)
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140NFI1 (Residues: 1-176; Coverage: 100%)
Gene names: ECBD_3472, thpR
Sequence domains: 2'-5' RNA ligase superfamily
Structure domains: Cyclic phosphodiesterase

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I911-2
Spacegroup: C2
Unit cell:
a: 102.15Å b: 73.47Å c: 77.61Å
α: 90° β: 128.34° γ: 90°
R-values:
R R work R free
0.171 0.169 0.208
Expression system: Escherichia coli BL21(DE3)