› 5lfj

X-ray diffraction
2.6Å resolution

Crystal Structure of the Bacterial Proteasome Activator Bpa of Mycobacterium tuberculosis

Released:
DOI: 10.2210/pdb5lfj/pdb
PDB entry 5lfj coloured by chain, front view.
PDB entry 5lfj coloured by chain, side view.
PDB entry 5lfj coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
Structural Analysis of the Bacterial Proteasome Activator Bpa in Complex with the 20S Proteasome.
Bolten M, Delley CL, Leibundgut M, Boehringer D, Ban N, Weber-Ban E
Structure 24 2138-2151 (2016)
PMID: 27839949

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161758 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bacterial proteasome activator Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 125 amino acids
Theoretical weight: 13.88 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WKX3 (Residues: 36-159; Coverage: 71%)
Gene names: MTCY13D12.14, Rv3780, bpa
Sequence domains: Bacterial proteasome activator

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: R32
Unit cell:
a: 101.378Å b: 101.378Å c: 311.246Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.228 0.224 0.293
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

AAA-ATPases in Protein Degradation.
Yedidi et al. (2017)
5 more

1 mention without citation

A particle size threshold governs diffusion and segregation of PAR-3 during cell polarization.
Chang et al. (2022)