› 5lhm

X-ray diffraction
1.31Å resolution

Crystal Structure of SafC from Myxococcus xanthus apo-Form

Released:
DOI: 10.2210/pdb5lhm/pdb
PDB entry 5lhm coloured by chain, front view.
PDB entry 5lhm coloured by chain, side view.
PDB entry 5lhm coloured by chain, top view.
Source organism: Myxococcus xanthus
Primary publication:
Functional and structural characterisation of a bacterial O-methyltransferase and factors determining regioselectivity.
Siegrist J, Netzer J, Mordhorst S, Karst L, Gerhardt S, Einsle O, Richter M, Andexer JN
FEBS Lett 591 312-321 (2017)
PMID: 27990630

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175885 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative O-methyltransferase Chain: A
Molecule details ›
Chain: A
Length: 233 amino acids
Theoretical weight: 26.26 KDa
Source organism: Myxococcus xanthus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q50859 (Residues: 1-220; Coverage: 100%)
Gene name: safC
Sequence domains: O-methyltransferase
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments

3 bound ligands:
Ligand MG 2 x MG
Ligand CL 1 x CL
Ligand BU3 5 x BU3
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P3121
Unit cell:
a: 64.558Å b: 64.558Å c: 118.163Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.178 0.177 0.196
Expression system: Escherichia coli BL21(DE3)

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Citations

12 citation in other articles

Genomics and biochemistry investigation on the metabolic pathway of milled wood and alkali lignin-derived aromatic metabolites of Comamonas serinivorans SP-35.
Zhu et al. (2018)
11 more

1 mention without citation

A structural metagenomics pipeline for examining the gut microbiome.
Walker et al. (2022)