› 5lkh

Electron Microscopy
3.46Å resolution

Cryo-EM structure of the Tc toxin TcdA1 in its pore state (obtained by flexible fitting)

Released:
DOI: 10.2210/pdb5lkh/pdb
PDB entry 5lkh coloured by chain, front view.
PDB entry 5lkh coloured by chain, side view.
PDB entry 5lkh coloured by chain, top view.
Source organism: Photorhabdus luminescens
Primary publication:
Membrane insertion of a Tc toxin in near-atomic detail.
Gatsogiannis C, Merino F, Prumbaum D, Roderer D, Leidreiter F, Meusch D, Raunser S
Nat Struct Mol Biol 23 884-890 (2016)
PMID: 27571177
Related structures: EMD-4068

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193207 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
TcdA1 Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 2516 amino acids
Theoretical weight: 283.23 KDa
Source organism: Photorhabdus luminescens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9RN43 (Residues: 1-2516; Coverage: 100%)
Gene names: tcdA, tcdA1
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.46Å
Relevant EMDB volumes: EMD-4068
Expression system: Escherichia coli

Quick links

Citations

15 review citations

Nanodiscs in Membrane Biochemistry and Biophysics.
Denisov et al. (2017)
14 more

3 mentions without citation

Common architecture of Tc toxins from human and insect pathogenic bacteria.
Leidreiter et al. (2019)
2 more