› 5llx

X-ray diffraction
2.8Å resolution

Bacteriophytochrome activated diguanylyl cyclase from Idiomarina species A28L with GTP bound

Released:
DOI: 10.2210/pdb5llx/pdb
PDB entry 5llx coloured by chain, front view.
PDB entry 5llx coloured by chain, side view.
PDB entry 5llx coloured by chain, top view.
Source organism: Idiomarina sp. A28L
Primary publication:
Long-range allosteric signaling in red light-regulated diguanylyl cyclases.
Gourinchas G, Etzl S, Göbl C, Vide U, Madl T, Winkler A
OpenAccess logo Sci Adv 3 e1602498 (2017)
PMID: 28275738

Function and Biology Details

Reaction catalysed: 
2 GTP = 2 diphosphate + cyclic di-3',5'-guanylate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123678 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
diguanylate cyclase Chains: A, B
Molecule details ›
Chains: A, B
Length: 685 amino acids
Theoretical weight: 78.16 KDa
Source organism: Idiomarina sp. A28L
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: F7RW09 (Residues: 3-683; Coverage: 100%)
Gene name: A28LD_0430
Sequence domains:

Ligands and Environments

4 bound ligands:
Ligand LBV 2 x LBV
Ligand CL 2 x CL
Ligand GTP 2 x GTP
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID30B
Spacegroup: P212121
Unit cell:
a: 50.73Å b: 78.64Å c: 452.04Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.223 0.27
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

10 review citations

Signal transduction in photoreceptor histidine kinases.
Möglich A. (2019)
9 more

6 mentions without citation

PAS domains in bacterial signal transduction.
Stuffle et al. (2021)
5 more