› 5log

X-ray diffraction
2.01Å resolution

Crystal Structure of SafC from Myxococcus xanthus bound to SAM

Released:
DOI: 10.2210/pdb5log/pdb
PDB entry 5log coloured by chain, front view.
PDB entry 5log coloured by chain, side view.
PDB entry 5log coloured by chain, top view.
Source organism: Myxococcus xanthus
Primary publication:
Functional and structural characterisation of a bacterial O-methyltransferase and factors determining regioselectivity.
Siegrist J, Netzer J, Mordhorst S, Karst L, Gerhardt S, Einsle O, Richter M, Andexer JN
FEBS Lett 591 312-321 (2017)
PMID: 27990630

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175886 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative O-methyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 233 amino acids
Theoretical weight: 26.26 KDa
Source organism: Myxococcus xanthus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q50859 (Residues: 1-220; Coverage: 100%)
Gene name: safC
Sequence domains: O-methyltransferase
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments

4 bound ligands:
Ligand SAH 1 x SAH
Ligand MG 2 x MG
Ligand CL 1 x CL
Ligand LDP 1 x LDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P21
Unit cell:
a: 60.84Å b: 51.017Å c: 72.677Å
α: 90° β: 98.58° γ: 90°
R-values:
R R work R free
0.178 0.176 0.212
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

A structural metagenomics pipeline for examining the gut microbiome.
Walker et al. (2022)
12 other articles

5 mentions without citation

Local Bacteriophage Delivery for Treatment and Prevention of Bacterial Infections.
Rotman et al. (2020)
4 more