5luf Citations

Functional asymmetry and electron flow in the bovine respirasome.

OpenAccess logo Elife 5 (2016)
Cited: 73 times
EuropePMC logo PMID: 27830641

Abstract

Respirasomes are macromolecular assemblies of the respiratory chain complexes I, III and IV in the inner mitochondrial membrane. We determined the structure of supercomplex I1III2IV1 from bovine heart mitochondria by cryo-EM at 9 Å resolution. Most protein-protein contacts between complex I, III and IV in the membrane are mediated by supernumerary subunits. Of the two Rieske iron-sulfur cluster domains in the complex III dimer, one is resolved, indicating that this domain is immobile and unable to transfer electrons. The central position of the active complex III monomer between complex I and IV in the respirasome is optimal for accepting reduced quinone from complex I over a short diffusion distance of 11 nm, and delivering reduced cytochrome c to complex IV. The functional asymmetry of complex III provides strong evidence for directed electron flow from complex I to complex IV through the active complex III monomer in the mammalian supercomplex.

Reviews - 5luf mentioned but not cited (3)

  1. Structure and mechanism of mitochondrial electron transport chain. Guo R, Gu J, Zong S, Wu M, Yang M. Biomed J 41 9-20 (2018)
  2. Structure and Mechanism of Respiratory III-IV Supercomplexes in Bioenergetic Membranes. Brzezinski P, Moe A, Ädelroth P. Chem Rev 121 9644-9673 (2021)
  3. The road to the structure of the mitochondrial respiratory chain supercomplex. Caruana NJ, Stroud DA. Biochem Soc Trans 48 621-629 (2020)

Articles - 5luf mentioned but not cited (3)



Reviews citing this publication (26)

  1. The assembly, regulation and function of the mitochondrial respiratory chain. Vercellino I, Sazanov LA. Nat Rev Mol Cell Biol 23 141-161 (2022)
  2. Clarifying the supercomplex: the higher-order organization of the mitochondrial electron transport chain. Letts JA, Sazanov LA. Nat Struct Mol Biol 24 800-808 (2017)
  3. Mitochondrial cytochrome c oxidase biogenesis: Recent developments. Timón-Gómez A, Nývltová E, Abriata LA, Vila AJ, Hosler J, Barrientos A. Semin Cell Dev Biol 76 163-178 (2018)
  4. Assembly of mammalian oxidative phosphorylation complexes I-V and supercomplexes. Signes A, Fernandez-Vizarra E. Essays Biochem 62 255-270 (2018)
  5. Building a complex complex: Assembly of mitochondrial respiratory chain complex I. Formosa LE, Dibley MG, Stroud DA, Ryan MT. Semin Cell Dev Biol 76 154-162 (2018)
  6. Mechanisms of mitochondrial respiratory adaptation. Bennett CF, Latorre-Muro P, Puigserver P. Nat Rev Mol Cell Biol 23 817-835 (2022)
  7. Metabolons, enzyme-enzyme assemblies that mediate substrate channeling, and their roles in plant metabolism. Zhang Y, Fernie AR. Plant Commun 2 100081 (2021)
  8. Why All the Fuss about Oxidative Phosphorylation (OXPHOS)? Xu Y, Xue D, Bankhead A, Neamati N. J Med Chem 63 14276-14307 (2020)
  9. Mitochondrial respiratory supercomplexes in mammalian cells: structural versus functional role. Javadov S, Jang S, Chapa-Dubocq XR, Khuchua Z, Camara AK. J Mol Med (Berl) 99 57-73 (2021)
  10. Molecular and Supramolecular Structure of the Mitochondrial Oxidative Phosphorylation System: Implications for Pathology. Nesci S, Trombetti F, Pagliarani A, Ventrella V, Algieri C, Tioli G, Lenaz G. Life (Basel) 11 242 (2021)
  11. Regulation and functional role of the electron transport chain supercomplexes. Cogliati S, Cabrera-Alarcón JL, Enriquez JA. Biochem Soc Trans 49 2655-2668 (2021)
  12. mtDNA Heteroplasmy at the Core of Aging-Associated Heart Failure. An Integrative View of OXPHOS and Mitochondrial Life Cycle in Cardiac Mitochondrial Physiology. Elorza AA, Soffia JP. Front Cell Dev Biol 9 625020 (2021)
  13. Stress-mediated generation of deleterious ROS in healthy individuals - role of cytochrome c oxidase. Ramzan R, Vogt S, Kadenbach B. J Mol Med (Berl) 98 651-657 (2020)
  14. Passing the Baton: Substrate Channelling in Respiratory Metabolism. Fernie AR, Zhang Y, Sweetlove LJ. Research (Wash D C) 2018 1539325 (2018)
  15. Mitochondrial Supercomplexes: Physiological Organization and Dysregulation in Age-Related Neurodegenerative Disorders. Novack GV, Galeano P, Castaño EM, Morelli L. Front Endocrinol (Lausanne) 11 600 (2020)
  16. Cytochrome c: Surfing Off of the Mitochondrial Membrane on the Tops of Complexes III and IV. Pérez-Mejías G, Guerra-Castellano A, Díaz-Quintana A, De la Rosa MA, Díaz-Moreno I. Comput Struct Biotechnol J 17 654-660 (2019)
  17. The importance of the membrane for biophysical measurements. Chorev DS, Robinson CV. Nat Chem Biol 16 1285-1292 (2020)
  18. Mitochondrial Respiratory Chain Supercomplexes: From Structure to Function. Guan S, Zhao L, Peng R. Int J Mol Sci 23 13880 (2022)
  19. Three-dimensional electron microscopy techniques for unravelling mitochondrial dysfunction in heart failure and identification of new pharmacological targets. Daghistani HM, Rajab BS, Kitmitto A. Br J Pharmacol 176 4340-4359 (2019)
  20. Research journey of respirasome. Wu M, Gu J, Zong S, Guo R, Liu T, Yang M. Protein Cell 11 318-338 (2020)
  21. The functional significance of mitochondrial respiratory chain supercomplexes. Kohler A, Barrientos A, Fontanesi F, Ott M. EMBO Rep 24 e57092 (2023)
  22. Tackling Dysfunction of Mitochondrial Bioenergetics in the Brain. Zanfardino P, Doccini S, Santorelli FM, Petruzzella V. Int J Mol Sci 22 8325 (2021)
  23. Mitochondrial Calcium Regulation of Cardiac Metabolism in Health and Disease. Balderas E, Lee SHJ, Rai NK, Mollinedo DM, Duron HE, Chaudhuri D. Physiology (Bethesda) 39 0 (2024)
  24. The Interplay among Subunit Composition, Cardiolipin Content, and Aggregation State of Bovine Heart Cytochrome c Oxidase. Sedlák E, Kožár T, Musatov A. Cells 9 E2588 (2020)
  25. Organization of the Respiratory Supercomplexes in Cells with Defective Complex III: Structural Features and Metabolic Consequences. Rugolo M, Zanna C, Ghelli AM. Life (Basel) 11 351 (2021)
  26. The Assembly of Super-Complexes in the Plant Chloroplast. Qin K, Fernie AR, Zhang Y. Biomolecules 11 1839 (2021)

Articles citing this publication (41)