› 5lw3

X-ray diffraction
1.19Å resolution

Azotobacter vinelandii Mannuronan C-5 epimerase AlgE6 A-module

Released:
DOI: 10.2210/pdb5lw3/pdb
PDB entry 5lw3 coloured by chain, front view.
PDB entry 5lw3 coloured by chain, side view.
PDB entry 5lw3 coloured by chain, top view.
Source organism: Azotobacter vinelandii
Entry author: Rothweiler U

Function and Biology Details

Reaction catalysed: 
(Mannuronan)-beta-D-mannuronate = (alginate)-alpha-L-guluronate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195632 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mannuronan C5-epimerase AlgE6 Chain: A
Molecule details ›
Chain: A
Length: 387 amino acids
Theoretical weight: 40.8 KDa
Source organism: Azotobacter vinelandii
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9ZFH0 (Residues: 1-385; Coverage: 44%)
Gene name: algE6
Sequence domains:
Structure domains: Single-stranded right-handed beta-helix, Pectin lyase-like

Ligands and Environments

3 bound ligands:
Ligand CL 1 x CL
Ligand B3P 1 x B3P
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: C2
Unit cell:
a: 100.884Å b: 63.672Å c: 73.075Å
α: 90° β: 97.16° γ: 90°
R-values:
R R work R free
0.145 0.144 0.16
Expression system: Escherichia coli

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