› 5lwj

Solution NMR

Solution NMR structure of the GTP binding Class II RNA aptamer-ligand-complex containing a protonated adenine nucleotide with a highly shifted pKa.

Released:
DOI: 10.2210/pdb5lwj/pdb
PDB entry 5lwj coloured by chain, ensemble of 10 models, front view.
PDB entry 5lwj coloured by chain, ensemble of 10 models, side view.
PDB entry 5lwj coloured by chain, ensemble of 10 models, top view.
Source organism: synthetic construct
Primary publication:
A Stably Protonated Adenine Nucleotide with a Highly Shifted pKa Value Stabilizes the Tertiary Structure of a GTP-Binding RNA Aptamer.
Wolter AC, Weickhmann AK, Nasiri AH, Hantke K, Ohlenschläger O, Wunderlich CH, Kreutz C, Duchardt-Ferner E, Wöhnert J
Angew Chem Int Ed Engl 56 401-404 (2017)
PMID: 27885761

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-196953 (preferred)
Entry contents:
1 distinct RNA molecule
Macromolecule:
GTP Class II RNA (34-MER) Chain: A
Molecule details ›
Chain: A
Length: 34 nucleotides
Theoretical weight: 11.02 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand GTP 1 x GTP
1 modified residue:
Ligand AP7 1 x AP7

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 84%
Refinement method: molecular dynamics
Expression system: Not provided

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Citations

6 review citations

Advancement in Salmonella Detection Methods: From Conventional to Electrochemical-Based Sensing Detection.
Awang et al. (2021)
5 more

2 mentions without citation

RNA structure refinement using NMR solvent accessibility data.
Hartlmüller et al. (2017)
1 more