› 5m2o

X-ray diffraction
1.26Å resolution

R. flavefaciens' third ScaB cohesin in complex with a group 1 dockerin

Released:
DOI: 10.2210/pdb5m2o/pdb
PDB entry 5m2o coloured by chain, front view.
PDB entry 5m2o coloured by chain, side view.
PDB entry 5m2o coloured by chain, top view.
Source organism: Ruminococcus flavefaciens FD-1
Primary publication:
Assembly of Ruminococcus flavefaciens cellulosome revealed by structures of two cohesin-dockerin complexes.
Bule P, Alves VD, Israeli-Ruimy V, Carvalho AL, Ferreira LMA, Smith SP, Gilbert HJ, Najmudin S, Bayer EA, Fontes CMGA
OpenAccess logo Sci Rep 7 759 (2017)
PMID: 28389644

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106043 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Cohesin domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 149 amino acids
Theoretical weight: 15.95 KDa
Source organism: Ruminococcus flavefaciens FD-1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0AEF4 (Residues: 399-544; Coverage: 7%)
Gene name: scaB
Structure domains: Immunoglobulin-like
Group I Dockerin Chain: B
Molecule details ›
Chain: B
Length: 96 amino acids
Theoretical weight: 10.28 KDa
Source organism: Ruminococcus flavefaciens FD-1
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

1 bound ligand:
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P212121
Unit cell:
a: 42.767Å b: 63.51Å c: 84.482Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.136 0.135 0.154
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Surfaceome and Proteosurfaceome in Parietal Monoderm Bacteria: Focus on Protein Cell-Surface Display.
Desvaux et al. (2018)
4 more

7 mentions without citation

Assembly of Ruminococcus flavefaciens cellulosome revealed by structures of two cohesin-dockerin complexes.
Bule et al. (2017)
6 more