› 5m3i

X-ray diffraction
2.17Å resolution

Macrodomain of Mycobacterium tuberculosis DarG

Released:
DOI: 10.2210/pdb5m3i/pdb
PDB entry 5m3i coloured by chain, front view.
PDB entry 5m3i coloured by chain, side view.
PDB entry 5m3i coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
The Toxin-Antitoxin System DarTG Catalyzes Reversible ADP-Ribosylation of DNA.
Jankevicius G, Ariza A, Ahel M, Ahel I
OpenAccess logo Mol Cell 64 1109-1116 (2016)
PMID: 27939941

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129456 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA ADP-ribosyl glycohydrolase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 165 amino acids
Theoretical weight: 18.07 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O53605 (Residues: 1-155; Coverage: 44%)
Gene names: Rv0060, darG
Sequence domains: Macro domain

Ligands and Environments

1 bound ligand:
Ligand CL 3 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P212121
Unit cell:
a: 68.84Å b: 75.45Å c: 116.12Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.212 0.251
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

38 review citations

PARPs and ADP-ribosylation: recent advances linking molecular functions to biological outcomes.
Gupte et al. (2017)
37 more