› 5m9z

Solution NMR

Second zinc-binding domain from yeast Pcf11

Released:
DOI: 10.2210/pdb5m9z/pdb
PDB entry 5m9z coloured by chain, ensemble of 15 models, front view.
PDB entry 5m9z coloured by chain, ensemble of 15 models, side view.
PDB entry 5m9z coloured by chain, ensemble of 15 models, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Distinct roles of Pcf11 zinc-binding domains in pre-mRNA 3'-end processing.
Guéguéniat J, Dupin AF, Stojko J, Beaurepaire L, Cianférani S, Mackereth CD, Minvielle-Sébastia L, Fribourg S
OpenAccess logo Nucleic Acids Res 45 10115-10131 (2017)
PMID: 28973460

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153821 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein PCF11 Chain: A
Molecule details ›
Chain: A
Length: 97 amino acids
Theoretical weight: 10.84 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P39081 (Residues: 530-626; Coverage: 16%)
Gene names: PCF11, YD9934.13C, YDR228C
Sequence domains: Pcf11, C-terminal domain

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 88%
Refinement method: simulated annealing
Expression system: Escherichia coli BL21

Quick links

Citations

2 review citations

Birth of a poly(A) tail: mechanisms and control of mRNA polyadenylation.
Rodríguez-Molina et al. (2023)
1 more

1 mention without citation

Distinct roles of Pcf11 zinc-binding domains in pre-mRNA 3'-end processing.
Guéguéniat et al. (2017)