5mdw

Electron Microscopy
3.06Å resolution

Structure of ArfA(A18T) and RF2 bound to the 70S ribosome (pre-accommodated state)

Released:
Source organism: Escherichia coli
Primary publication:
Translational termination without a stop codon.
Science 354 1437-1440 (2016)
PMID: 27934701
Related structures: EMD-3490

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 58-mer (preferred)
PDBe Complex ID:
PDB-CPX-135474 (preferred)
Entry contents:
53 distinct polypeptide molecules
5 distinct RNA molecules
Macromolecules (58 distinct):
Alternative ribosome-rescue factor A Chain: 6
Molecule details ›
Chain: 6
Length: 61 amino acids
Theoretical weight: 7.15 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P36675 (Residues: 1-60; Coverage: 83%)
Gene names: JW3253, arfA, b4550, yhdL
Sequence domains: Alternative ribosome-rescue factor A
Peptide chain release factor RF2 Chain: 7
Molecule details ›
Chain: 7
Length: 365 amino acids
Theoretical weight: 41.31 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P07012 (Residues: 1-365; Coverage: 100%)
Gene names: JW5847, b2891, prfB, supK
Sequence domains:
Large ribosomal subunit protein uL2 Chain: B
Large ribosomal subunit protein uL3 Chain: C
Large ribosomal subunit protein uL4 Chain: D
Large ribosomal subunit protein uL5 Chain: E
Large ribosomal subunit protein uL6 Chain: F
Large ribosomal subunit protein bL9 Chain: G
Large ribosomal subunit protein uL10 Chain: H
Large ribosomal subunit protein uL11 Chain: I
Large ribosomal subunit protein uL13 Chain: J
Large ribosomal subunit protein uL14 Chain: K
Large ribosomal subunit protein uL15 Chain: L
Large ribosomal subunit protein uL16 Chain: M
Large ribosomal subunit protein bL17 Chain: N
Large ribosomal subunit protein uL18 Chain: O
Large ribosomal subunit protein bL19 Chain: P
Large ribosomal subunit protein bL20 Chain: Q
Large ribosomal subunit protein bL21 Chain: R
Large ribosomal subunit protein uL22 Chain: S
Large ribosomal subunit protein uL23 Chain: T
Large ribosomal subunit protein uL24 Chain: U
Large ribosomal subunit protein bL25 Chain: V
Large ribosomal subunit protein bL27 Chain: W
Large ribosomal subunit protein bL28 Chain: X
Large ribosomal subunit protein uL29 Chain: Y
Large ribosomal subunit protein uL30 Chain: Z
Large ribosomal subunit protein bL31 Chain: a
Large ribosomal subunit protein bL32 Chain: b
Large ribosomal subunit protein bL33 Chain: c
Large ribosomal subunit protein bL34 Chain: d
Large ribosomal subunit protein bL35 Chain: e
Large ribosomal subunit protein bL36A Chain: f
Small ribosomal subunit protein uS2 Chain: g
Small ribosomal subunit protein uS3 Chain: h
Small ribosomal subunit protein uS4 Chain: i
Small ribosomal subunit protein uS5 Chain: j
Small ribosomal subunit protein bS6 Chain: k
Small ribosomal subunit protein uS7 Chain: l
Small ribosomal subunit protein uS8 Chain: m
Small ribosomal subunit protein uS9 Chain: n
Small ribosomal subunit protein uS10 Chain: o
Small ribosomal subunit protein uS11 Chain: p
Small ribosomal subunit protein uS12 Chain: q
Small ribosomal subunit protein uS13 Chain: r
Small ribosomal subunit protein uS14 Chain: s
Small ribosomal subunit protein uS15 Chain: t
Small ribosomal subunit protein bS16 Chain: u
Small ribosomal subunit protein uS17 Chain: v
Small ribosomal subunit protein bS18 Chain: w
Small ribosomal subunit protein uS19 Chain: x
Small ribosomal subunit protein bS20 Chain: y
Small ribosomal subunit protein bS21 Chain: z
23S ribosomal RNA Chain: 1
16S ribosomal RNA Chain: 2
5S ribosomal RNA Chain: 3
Messenger RNA Chain: 4
fMet-NH-tRNA(fMet) Chain: 5

Ligands and Environments

Experiments and Validation Details

Entry percentile scores
Resolution: 3.06Å
Relevant EMDB volumes: EMD-3490
Expression system: Escherichia coli