5mg3

Electron Microscopy
14Å resolution

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-142800 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Protein translocase subunit SecY Chain: Y
Molecule details ›
Chain: Y
Length: 458 amino acids
Theoretical weight: 50.41 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AGA2 (Residues: 1-443; Coverage: 100%)
Gene names: JW3262, b3300, prlA, secY
Sequence domains: SecY
Protein translocase subunit SecE Chain: E
Molecule details ›
Chain: E
Length: 140 amino acids
Theoretical weight: 15.25 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AG96 (Residues: 3-127; Coverage: 98%)
Gene names: JW3944, b3981, prlG, secE
Sequence domains: SecE/Sec61-gamma subunits of protein translocation complex
Protein-export membrane protein SecG Chain: G
Molecule details ›
Chain: G
Length: 136 amino acids
Theoretical weight: 14.33 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AG99 (Residues: 1-110; Coverage: 100%)
Gene names: JW3142, b3175, secG
Sequence domains: Preprotein translocase SecG subunit
Protein translocase subunit SecD Chain: D
Molecule details ›
Chain: D
Length: 622 amino acids
Theoretical weight: 67.69 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AG90 (Residues: 2-615; Coverage: 100%)
Gene names: JW0398, b0408, secD
Sequence domains:
Protein translocase subunit SecF Chain: F
Molecule details ›
Chain: F
Length: 323 amino acids
Theoretical weight: 35.41 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AG93 (Residues: 1-323; Coverage: 100%)
Gene names: JW0399, b0409, secF
Sequence domains:
Membrane protein insertase YidC Chain: C
Molecule details ›
Chain: C
Length: 559 amino acids
Theoretical weight: 62.66 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P25714 (Residues: 2-548; Coverage: 100%)
Gene names: JW3683, b3705, yidC
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 14Å
Relevant EMDB volumes: EMD-3506
Expression system: Escherichia coli