› 5mj3

X-ray diffraction
1.74Å resolution

INTERLEUKIN-23 COMPLEX WITH AN ANTAGONISTIC ALPHABODY, CRYSTAL FORM 1

Released:
DOI: 10.2210/pdb5mj3/pdb
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PDB entry 5mj3 coloured by chain, front view.
PDB entry 5mj3 coloured by chain, side view.
PDB entry 5mj3 coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of IL-23 antagonism by an Alphabody protein scaffold.
Desmet J, Verstraete K, Bloch Y, Lorent E, Wen Y, Devreese B, Vandenbroucke K, Loverix S, Hettmann T, Deroo S, Somers K, Henderikx P, Lasters I, Savvides SN
OpenAccess logo Nat Commun 5 5237 (2014)
PMID: 25354530

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151475 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (4 distinct):
Interleukin-12 subunit beta Chain: A
Molecule details ›
Chain: A
Length: 306 amino acids
Theoretical weight: 34.74 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P29460 (Residues: 23-328; Coverage: 100%)
Gene names: IL12B, NKSF2
Sequence domains: Cytokine interleukin-12p40 C-terminus
Structure domains: Immunoglobulins
Interleukin-23 subunit alpha Chain: B
Molecule details ›
Chain: B
Length: 179 amino acids
Theoretical weight: 19.81 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9NPF7 (Residues: 20-189; Coverage: 100%)
Gene names: IL23A, SGRF, UNQ2498/PRO5798
Sequence domains: Interleukin 23 subunit alpha
Structure domains: Growth Hormone; Chain: A;
ALPHABODY MA12 Chain: C

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
3 bound ligands:
Ligand PGE 2 x PGE
Ligand PG4 1 x PG4
Ligand NA 2 x NA
1 modified residue:
Ligand YCM 1 x YCM

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21
Unit cell:
a: 56.96Å b: 56.74Å c: 100.07Å
α: 90° β: 99.73° γ: 90°
R-values:
R R work R free
0.165 0.165 0.198
Expression systems:
  • Homo sapiens
  • Escherichia coli BL21(DE3)

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Citations

13 review citations

Cytokines in psoriasis.
Baliwag et al. (2015)
12 more

5 mentions without citation

Structural Activation of Pro-inflammatory Human Cytokine IL-23 by Cognate IL-23 Receptor Enables Recruitment of the Shared Receptor IL-12Rβ1.
Bloch et al. (2018)
4 more

PDB-REDO

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