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Pink-beam serial crystallography.

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Meents A, Wiedorn MO, Srajer V, Henning R, Sarrou I, Bergtholdt J, Barthelmess M, Reinke PYA, Dierksmeyer D, Tolstikova A, Schaible S, Messerschmidt M, Ogata CM, Kissick DJ, Taft MH, Manstein DJ, Lieske J, Oberthuer D, Fischetti RF, Chapman HN
OpenAccess logo Nat Commun 8 1281 (2017)
Related entries: 5mjm, 5mjp, 5mjq, 5o7m

Cited: 35 times
EuropePMC logo PMID: 29097720

Abstract

Serial X-ray crystallography allows macromolecular structure determination at both X-ray free electron lasers (XFELs) and, more recently, synchrotron sources. The time resolution for serial synchrotron crystallography experiments has been limited to millisecond timescales with monochromatic beams. The polychromatic, "pink", beam provides a more than two orders of magnitude increased photon flux and hence allows accessing much shorter timescales in diffraction experiments at synchrotron sources. Here we report the structure determination of two different protein samples by merging pink-beam diffraction patterns from many crystals, each collected with a single 100 ps X-ray pulse exposure per crystal using a setup optimized for very low scattering background. In contrast to experiments with monochromatic radiation, data from only 50 crystals were required to obtain complete datasets. The high quality of the diffraction data highlights the potential of this method for studying irreversible reactions at sub-microsecond timescales using high-brightness X-ray facilities.

Articles - 5mjl mentioned but not cited (3)

  1. Pink-beam serial crystallography. Meents A, Wiedorn MO, Srajer V, Henning R, Sarrou I, Bergtholdt J, Barthelmess M, Reinke PYA, Dierksmeyer D, Tolstikova A, Schaible S, Messerschmidt M, Ogata CM, Kissick DJ, Taft MH, Manstein DJ, Lieske J, Oberthuer D, Fischetti RF, Chapman HN. Nat Commun 8 1281 (2017)
  2. High-viscosity injector-based pink-beam serial crystallography of microcrystals at a synchrotron radiation source. Martin-Garcia JM, Zhu L, Mendez D, Lee MY, Chun E, Li C, Hu H, Subramanian G, Kissick D, Ogata C, Henning R, Ishchenko A, Dobson Z, Zhang S, Weierstall U, Spence JCH, Fromme P, Zatsepin NA, Fischetti RF, Cherezov V, Liu W. IUCrJ 6 412-425 (2019)
  3. Serial macromolecular crystallography at ALBA Synchrotron Light Source. Martin-Garcia JM, Botha S, Hu H, Jernigan R, Castellví A, Lisova S, Gil F, Calisto B, Crespo I, Roy-Chowdhury S, Grieco A, Ketawala G, Weierstall U, Spence J, Fromme P, Zatsepin N, Boer DR, Carpena X. J Synchrotron Radiat 29 896-907 (2022)


Reviews citing this publication (11)

  1. X-Ray Free-Electron Lasers for the Structure and Dynamics of Macromolecules. Chapman HN. Annu Rev Biochem 88 35-58 (2019)
  2. Time-Resolved Macromolecular Crystallography at Pulsed X-ray Sources. Schmidt M. Int J Mol Sci 20 E1401 (2019)
  3. Mapping Enzyme Landscapes by Time-Resolved Crystallography with Synchrotron and X-Ray Free Electron Laser Light. Wilson MA. Annu Rev Biophys 51 79-98 (2022)
  4. Macromolecular movies, storybooks written by nature. Schmidt M. Biophys Rev 13 1191-1197 (2021)
  5. Serial Crystallography for Structure-Based Drug Discovery. Zhu L, Chen X, Abola EE, Jing L, Liu W. Trends Pharmacol Sci 41 830-839 (2020)
  6. Dynamic Structural Biology Experiments at XFEL or Synchrotron Sources. Aller P, Orville AM. Methods Mol Biol 2305 203-228 (2021)
  7. Emerging Time-Resolved X-Ray Diffraction Approaches for Protein Dynamics. Hekstra DR. Annu Rev Biophys 52 255-274 (2023)
  8. Exploring the structural aspects and therapeutic perspectives of cyanobacterial phycobiliproteins. Patel SN, Sonani RR, Roy D, Singh NK, Subudhi S, Pabbi S, Madamwar D. 3 Biotech 12 224 (2022)
  9. Observations on Laue diffraction within synchrotron radiation and neutron macromolecular crystallography research and developments. Helliwell JR. Struct Dyn 10 061301 (2023)
  10. SARS-CoV-2 proteins structural studies using synchrotron radiation. Kosenko M, Onkhonova G, Susloparov I, Ryzhikov A. Biophys Rev 15 1185-1194 (2023)
  11. The Extremely Brilliant Source storage ring of the European Synchrotron Radiation Facility. Raimondi P, Benabderrahmane C, Berkvens P, Biasci JC, Borowiec P, Bouteille JF, Brochard T, Brookes NB, Carmignani N, Carver LR, Chaize JM, Chavanne J, Checchia S, Chushkin Y, Cianciosi F, Di Michiel M, Dimper R, D'Elia A, Einfeld D, Ewald F, Farvacque L, Goirand L, Hardy L, Jacob J, Jolly L, Krisch M, Le Bec G, Leconte I, Liuzzo SM, Maccarrone C, Marchial T, Martin D, Mezouar M, Nevo C, Perron T, Plouviez E, Reichert H, Renaud P, Revol JL, Roche B, Scheidt KB, Serriere V, Sette F, Susini J, Torino L, Versteegen R, White S, Zontone F. Commun Phys 6 82 (2023)

Articles citing this publication (21)

  1. Radiation damage and dose limits in serial synchrotron crystallography at cryo- and room temperatures. de la Mora E, Coquelle N, Bury CS, Rosenthal M, Holton JM, Carmichael I, Garman EF, Burghammer M, Colletier JP, Weik M. Proc Natl Acad Sci U S A 117 4142-4151 (2020)
  2. Fast and accurate data collection for macromolecular crystallography using the JUNGFRAU detector. Leonarski F, Redford S, Mozzanica A, Lopez-Cuenca C, Panepucci E, Nass K, Ozerov D, Vera L, Olieric V, Buntschu D, Schneider R, Tinti G, Froejdh E, Diederichs K, Bunk O, Schmitt B, Wang M. Nat Methods 15 799-804 (2018)
  3. Sample manipulation and data assembly for robust microcrystal synchrotron crystallography. Guo G, Fuchs MR, Shi W, Skinner J, Berman E, Ogata CM, Hendrickson WA, McSweeney S, Liu Q. IUCrJ 5 238-246 (2018)
  4. Crystal-on-crystal chips for in situ serial diffraction at room temperature. Ren Z, Ayhan M, Bandara S, Bowatte K, Kumarapperuma I, Gunawardana S, Shin H, Wang C, Zeng X, Yang X. Lab Chip 18 2246-2256 (2018)
  5. pinkIndexer - a universal indexer for pink-beam X-ray and electron diffraction snapshots. Gevorkov Y, Barty A, Brehm W, White TA, Tolstikova A, Wiedorn MO, Meents A, Grigat RR, Chapman HN, Yefanov O. Acta Crystallogr A Found Adv 76 121-131 (2020)
  6. Entering an era of dynamic structural biology…. Orville AM. BMC Biol 16 55 (2018)
  7. Serial femtosecond and serial synchrotron crystallography can yield data of equivalent quality: A systematic comparison. Mehrabi P, Bücker R, Bourenkov G, Ginn HM, von Stetten D, Müller-Werkmeister HM, Kuo A, Morizumi T, Eger BT, Ou WL, Oghbaey S, Sarracini A, Besaw JE, Pare-Labrosse O, Meier S, Schikora H, Tellkamp F, Marx A, Sherrell DA, Axford D, Owen RL, Ernst OP, Pai EF, Schulz EC, Miller RJD. Sci Adv 7 eabf1380 (2021)
  8. A shared vision for macromolecular crystallography over the next five years. Förster A, Schulze-Briese C. Struct Dyn 6 064302 (2019)
  9. Rapid and efficient room-temperature serial synchrotron crystallography using the CFEL TapeDrive. Zielinski KA, Prester A, Andaleeb H, Bui S, Yefanov O, Catapano L, Henkel A, Wiedorn MO, Lorbeer O, Crosas E, Meyer J, Mariani V, Domaracky M, White TA, Fleckenstein H, Sarrou I, Werner N, Betzel C, Rohde H, Aepfelbacher M, Chapman HN, Perbandt M, Steiner RA, Oberthuer D. IUCrJ 9 778-791 (2022)
  10. Dispersion-Based Fresh-Slice Scheme for Free-Electron Lasers. Guetg MW, Lutman AA, Ding Y, Maxwell TJ, Huang Z. Phys Rev Lett 120 264802 (2018)
  11. Time-resolved β-lactam cleavage by L1 metallo-β-lactamase. Wilamowski M, Sherrell DA, Kim Y, Lavens A, Henning RW, Lazarski K, Shigemoto A, Endres M, Maltseva N, Babnigg G, Burdette SC, Srajer V, Joachimiak A. Nat Commun 13 7379 (2022)
  12. A unifying Bayesian framework for merging X-ray diffraction data. Dalton KM, Greisman JB, Hekstra DR. Nat Commun 13 7764 (2022)
  13. C-phycocyanin as a highly attractive model system in protein crystallography: unique crystallization properties and packing-diversity screening. Sarrou I, Feiler CG, Falke S, Peard N, Yefanov O, Chapman H. Acta Crystallogr D Struct Biol 77 224-236 (2021)
  14. Doses for experiments with microbeams and microcrystals: Monte Carlo simulations in RADDOSE-3D. Dickerson JL, Garman EF. Protein Sci 30 8-19 (2021)
  15. High-throughput in situ experimental phasing. Lawrence JM, Orlans J, Evans G, Orville AM, Foadi J, Aller P. Acta Crystallogr D Struct Biol 76 790-801 (2020)
  16. Compact all-optical precision-tunable narrowband hard Compton X-ray source. Brümmer T, Bohlen S, Grüner F, Osterhoff J, Põder K. Sci Rep 12 16017 (2022)
  17. Complementarity of neutron, XFEL and synchrotron crystallography for defining the structures of metalloenzymes at room temperature. Moreno-Chicano T, Carey LM, Axford D, Beale JH, Doak RB, Duyvesteyn HME, Ebrahim A, Henning RW, Monteiro DCF, Myles DA, Owada S, Sherrell DA, Straw ML, Šrajer V, Sugimoto H, Tono K, Tosha T, Tews I, Trebbin M, Strange RW, Weiss KL, Worrall JAR, Meilleur F, Owen RL, Ghiladi RA, Hough MA. IUCrJ 9 610-624 (2022)
  18. A simple goniometer-compatible flow cell for serial synchrotron X-ray crystallography. Ghosh S, Zorić D, Dahl P, Bjelčić M, Johannesson J, Sandelin E, Borjesson P, Björling A, Banacore A, Edlund P, Aurelius O, Milas M, Nan J, Shilova A, Gonzalez A, Mueller U, Brändén G, Neutze R. J Appl Crystallogr 56 449-460 (2023)
  19. Crystal diffraction prediction and partiality estimation using Gaussian basis functions. Brehm W, White T, Chapman HN. Acta Crystallogr A Found Adv 79 145-162 (2023)
  20. Data of pink-beam serial synchrotron crystallography at the Pohang Light Source II. Kim Y, Nam KH. Data Brief 52 109811 (2024)
  21. Depth-of-field extension in optical imaging for rapid crystal screening. Li C, Ding C, Li M, Rong J, Florian H, Simpson G. Acta Crystallogr D Struct Biol 77 463-470 (2021)


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