› 5mko

X-ray diffraction
2.65Å resolution

[2Fe-2S] cluster containing TtuA in complex with AMP.

Released:
DOI: 10.2210/pdb5mko/pdb
PDB entry 5mko coloured by chain, front view.
PDB entry 5mko coloured by chain, side view.
PDB entry 5mko coloured by chain, top view.
Source organism: Pyrococcus horikoshii OT3
Primary publication:
Nonredox thiolation in tRNA occurring via sulfur activation by a [4Fe-4S] cluster.
Arragain S, Bimai O, Legrand P, Caillat S, Ravanat JL, Touati N, Binet L, Atta M, Fontecave M, Golinelli-Pimpaneau B
Proc Natl Acad Sci U S A 114 7355-7360 (2017)
PMID: 28655838

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129662 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
tRNA-5-methyluridine(54) 2-sulfurtransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 313 amino acids
Theoretical weight: 35.92 KDa
Source organism: Pyrococcus horikoshii OT3
Expression system: Escherichia coli
UniProt:
  • Canonical: O58038 (Residues: 1-310; Coverage: 100%)
Gene names: PH0300, ttuA
Sequence domains:
Structure domains: HUPs

Ligands and Environments

3 bound ligands:
Ligand AMP 2 x AMP
Ligand ZN 4 x ZN
Ligand FES 2 x FES
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P212121
Unit cell:
a: 68.86Å b: 72.32Å c: 128.28Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.211 0.239
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Transfer RNA Modification Enzymes from Thermophiles and Their Modified Nucleosides in tRNA.
Hori et al. (2018)
6 more

3 mentions without citation

Nonredox thiolation in tRNA occurring via sulfur activation by a [4Fe-4S] cluster.
Arragain et al. (2017)
2 more