› 5mnu

X-ray diffraction
1.56Å resolution

OXA-10 Avibactam complex with bound bromide

Released:
DOI: 10.2210/pdb5mnu/pdb
PDB entry 5mnu coloured by chain, front view.
PDB entry 5mnu coloured by chain, side view.
PDB entry 5mnu coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
13C-Carbamylation as a mechanistic probe for the inhibition of class D β-lactamases by avibactam and halide ions.
Lohans CT, Wang DY, Jorgensen C, Cahill ST, Clifton IJ, McDonough MA, Oswin HP, Spencer J, Domene C, Claridge TDW, Brem J, Schofield CJ
Org Biomol Chem 15 6024-6032 (2017)
PMID: 28678295

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146958 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-lactamase OXA-10 Chains: A, B
Molecule details ›
Chains: A, B
Length: 247 amino acids
Theoretical weight: 27.66 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P14489 (Residues: 20-265; Coverage: 100%)
Gene names: OXA-10, bla, oxa10, pse2
Sequence domains: Penicillin binding protein transpeptidase domain
Structure domains: DD-peptidase/beta-lactamase superfamily

Ligands and Environments

4 bound ligands:
Ligand NXL 2 x NXL
Ligand GOL 7 x GOL
Ligand BR 6 x BR
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P212121
Unit cell:
a: 48.771Å b: 101.421Å c: 127.221Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.185 0.208
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

β-Lactamases and β-Lactamase Inhibitors in the 21st Century.
Tooke et al. (2019)
2 more