5mpe

Electron Microscopy
4.5Å resolution

26S proteasome in presence of ATP (s2)

Released:

Function and Biology Details

Reaction catalysed:
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Biochemical function:
Cellular component:

Structure analysis Details

Assemblies composition:
hetero tridecamer (preferred)
hetero dodecamer
Assembly name:
PDBe Complex ID:
PDB-CPX-517933 (preferred)
Entry contents:
13 distinct polypeptide molecules
Macromolecules (13 distinct):
26S proteasome regulatory subunit RPN10 Chain: W
Molecule details ›
Chain: W
Length: 268 amino acids
Theoretical weight: 29.78 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P38886 (Residues: 1-268; Coverage: 100%)
Gene names: MCB1, RPN10, SUN1, YHR200W
Sequence domains: von Willebrand factor type A domain
Ubiquitin carboxyl-terminal hydrolase RPN11 Chain: V
Molecule details ›
Chain: V
Length: 306 amino acids
Theoretical weight: 34.44 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P43588 (Residues: 1-306; Coverage: 100%)
Gene names: MPR1, RPN11, YFR004W
Sequence domains: JAB1/Mov34/MPN/PAD-1 ubiquitin protease
26S proteasome regulatory subunit RPN12 Chain: T
26S proteasome regulatory subunit RPN13 Chain: X
26S proteasome complex subunit SEM1 Chain: Y
26S proteasome regulatory subunit RPN1 Chain: Z
26S proteasome regulatory subunit RPN2 Chain: N
26S proteasome regulatory subunit RPN3 Chain: S
26S proteasome regulatory subunit RPN5 Chain: P
26S proteasome regulatory subunit RPN6 Chain: Q
26S proteasome regulatory subunit RPN7 Chain: R
26S proteasome regulatory subunit RPN8 Chain: U
26S proteasome regulatory subunit RPN9 Chain: O

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.5Å
Relevant EMDB volumes: EMD-3535