5ms0

Electron Microscopy
9.8Å resolution

pseudo-atomic model of the RNA polymerase lambda-based antitermination complex solved by cryo-EM

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetradecamer (preferred)
PDBe Complex ID:
PDB-CPX-119033 (preferred)
Entry contents:
9 distinct polypeptide molecules
2 distinct DNA molecules
2 distinct RNA molecules
Macromolecules (13 distinct):
Antitermination protein N Chain: N
Molecule details ›
Chain: N
Length: 85 amino acids
Theoretical weight: 9.88 KDa
Source organism: Lambdavirus lambda
Expression system: Escherichia coli
UniProt:
  • Canonical: P03045 (Residues: 3-86; Coverage: 79%)
Gene names: N, lambdap49
Sequence domains: 36-mer N-terminal peptide of the N protein (N36)
DNA-directed RNA polymerase subunit alpha Chains: A, B
Molecule details ›
Chains: A, B
Length: 329 amino acids
Theoretical weight: 36.56 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A7Z4 (Residues: 1-329; Coverage: 100%)
Gene names: JW3257, b3295, pez, phs, rpoA, sez
Sequence domains:
DNA-directed RNA polymerase subunit beta Chain: C
DNA-directed RNA polymerase subunit beta' Chain: D
Small ribosomal subunit protein uS10 Chain: E
Transcription termination/antitermination protein NusG Chain: F
Transcription antitermination protein NusB Chain: L
Transcription termination/antitermination protein NusA Chain: M
DNA-directed RNA polymerase subunit omega Chain: O
DNAI Chain: I
DNAII Chain: J
nascent RNA Chain: R
RNA transcription bubble Chain: H

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 9.8Å
Relevant EMDB volumes: EMD-3561
Expression system: Escherichia coli