Function and Biology Details
Reaction catalysed:
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
- Transcription antitermination protein, NusG
- Small ribosomal subunit protein uS10
- DNA-directed RNA polymerase, insert domain superfamily
- DNA-directed RNA polymerase, RpoA/D/Rpb3-type
- RNA polymerase, RBP11-like subunit
- DNA-directed RNA polymerase, alpha subunit
- DNA-directed RNA polymerase, omega subunit
- NusB antitermination factor
53 more domains
Structure analysis Details
Assembly composition:
hetero tetradecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-119033 (preferred)
Entry contents:
9 distinct polypeptide molecules
2 distinct DNA molecules
2 distinct RNA molecules
2 distinct DNA molecules
2 distinct RNA molecules
Macromolecules (13 distinct):
Antitermination protein N
Molecule details ›
Chain: N
Length: 85 amino acids
Theoretical weight: 9.88 KDa
Source organism: Lambdavirus lambda
Expression system: Escherichia coli
UniProt:
Sequence domains: 36-mer N-terminal peptide of the N protein (N36)
Length: 85 amino acids
Theoretical weight: 9.88 KDa
Source organism: Lambdavirus lambda
Expression system: Escherichia coli
UniProt:
- Canonical:
P03045 (Residues: 3-86; Coverage: 79%)
Sequence domains: 36-mer N-terminal peptide of the N protein (N36)
DNA-directed RNA polymerase subunit alpha
Molecule details ›
DNA-directed RNA polymerase subunit beta
Molecule details ›
DNA-directed RNA polymerase subunit beta'
Molecule details ›
Small ribosomal subunit protein uS10
Molecule details ›
Chain: E
Length: 100 amino acids
Theoretical weight: 11.34 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
Sequence domains: Ribosomal protein S10p/S20e
Length: 100 amino acids
Theoretical weight: 11.34 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
- Canonical: P0A7R5 (Residues: 4-103; Coverage: 97%)
Sequence domains: Ribosomal protein S10p/S20e
Transcription termination/antitermination protein NusG
Molecule details ›
Chain: F
Length: 183 amino acids
Theoretical weight: 20.69 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
Sequence domains:
Length: 183 amino acids
Theoretical weight: 20.69 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
- Canonical: P0AFG0 (Residues: 1-181; Coverage: 100%)
Sequence domains:
Transcription antitermination protein NusB
Molecule details ›
Chain: L
Length: 139 amino acids
Theoretical weight: 15.71 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
Sequence domains: NusB family
Length: 139 amino acids
Theoretical weight: 15.71 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
- Canonical: P0A780 (Residues: 1-139; Coverage: 100%)
Sequence domains: NusB family
Transcription termination/antitermination protein NusA
Molecule details ›
Chain: M
Length: 497 amino acids
Theoretical weight: 55.06 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
Sequence domains:
Length: 497 amino acids
Theoretical weight: 55.06 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
- Canonical: P0AFF6 (Residues: 1-495; Coverage: 100%)
Sequence domains:
DNA-directed RNA polymerase subunit omega
Molecule details ›
Chain: O
Length: 91 amino acids
Theoretical weight: 10.25 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
Sequence domains: RNA polymerase Rpb6
Length: 91 amino acids
Theoretical weight: 10.25 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
- Canonical: P0A800 (Residues: 1-91; Coverage: 100%)
Sequence domains: RNA polymerase Rpb6
