5ms2

X-ray diffraction
2.47Å resolution

Crystal structure of the Legionella pneumophila effector protein RavZ in complex with human LC3B

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-178656 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Cysteine protease RavZ Chain: A
Molecule details ›
Chain: A
Length: 433 amino acids
Theoretical weight: 48.52 KDa
Source organism: Legionella pneumophila subsp. pneumophila str. Philadelphia 1
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q5ZUV9 (Residues: 1-431; Coverage: 86%)
Gene names: lpg1683, ravZ
Sequence domains: RavZ C-terminal PI3P-binding domain
Microtubule-associated protein 1 light chain 3 beta Chain: B
Molecule details ›
Chain: B
Length: 124 amino acids
Theoretical weight: 14.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9GZQ8 (Residues: 1-119; Coverage: 95%)
Gene names: MAP1ALC3, MAP1LC3B
Sequence domains: Autophagy protein Atg8 ubiquitin like
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P21
Unit cell:
a: 51.55Å b: 69.55Å c: 90.17Å
α: 90° β: 101.08° γ: 90°
R-values:
R R work R free
0.18 0.177 0.238
Expression system: Escherichia coli BL21