5muy

X-ray diffraction
1.99Å resolution

Structure of a C-terminal domain of a reptarenavirus L protein with m7GTP

Released:

Function and Biology Details

Reaction catalysed:
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
PDBe Complex ID:
PDB-CPX-125339 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA-directed RNA polymerase L Chains: A, B
Molecule details ›
Chains: A, B
Length: 328 amino acids
Theoretical weight: 37.25 KDa
Source organism: CAS virus
Expression system: Escherichia coli
UniProt:
  • Canonical: J7HBG8 (Residues: 1721-2046; Coverage: 16%)
Gene name: L

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 76.34Å b: 76.601Å c: 116.06Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.225 0.222 0.276
Expression system: Escherichia coli