5my1

Electron Microscopy
7.6Å resolution

E. coli expressome

Released:
Source organism: Escherichia coli K-12
Primary publication:
Architecture of a transcribing-translating expressome.
Science 356 194-197 (2017)
PMID: 28408604
Related structures: EMD-3580

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 26-mer (preferred)
PDBe Complex ID:
PDB-CPX-249905 (preferred)
Entry contents:
24 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (25 distinct):
Small ribosomal subunit protein uS3 Chain: C
Molecule details ›
Chain: C
Length: 232 amino acids
Theoretical weight: 25.9 KDa
Source organism: Escherichia coli K-12
UniProt:
  • Canonical: P0A7V3 (Residues: 2-233; Coverage: 100%)
Gene names: JW3276, b3314, rpsC
Sequence domains:
Small ribosomal subunit protein uS4 Chain: D
Molecule details ›
Chain: D
Length: 205 amino acids
Theoretical weight: 23.38 KDa
Source organism: Escherichia coli K-12
UniProt:
  • Canonical: P0A7V8 (Residues: 2-206; Coverage: 100%)
Gene names: JW3258, b3296, ramA, rpsD
Sequence domains:
Small ribosomal subunit protein uS5 Chain: E
Small ribosomal subunit protein bS6 Chain: F
Small ribosomal subunit protein uS7 Chain: G
Small ribosomal subunit protein uS8 Chain: H
Small ribosomal subunit protein uS9 Chain: I
Small ribosomal subunit protein uS10 Chain: J
Small ribosomal subunit protein uS11 Chain: K
Small ribosomal subunit protein uS12 Chain: L
Small ribosomal subunit protein uS13 Chain: M
Small ribosomal subunit protein uS14 Chain: N
Small ribosomal subunit protein uS15 Chain: O
Small ribosomal subunit protein bS16 Chain: P
Small ribosomal subunit protein uS17 Chain: Q
Small ribosomal subunit protein bS18 Chain: R
Small ribosomal subunit protein uS19 Chain: S
Small ribosomal subunit protein bS20 Chain: T
Small ribosomal subunit protein uS2 Chain: B
Small ribosomal subunit protein bS21 Chain: U
DNA-directed RNA polymerase subunit alpha Chains: V, W
DNA-directed RNA polymerase subunit beta Chain: X
DNA-directed RNA polymerase subunit beta' Chain: Y
DNA-directed RNA polymerase subunit omega Chain: Z
16S ribosomal RNA Chain: A

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 7.6Å
Relevant EMDB volumes: EMD-3580
Expression system: Escherichia coli