› 5n3n

X-ray diffraction
1.22Å resolution

cAMP-dependent Protein Kinase A from Cricetulus griseus in complex with fragment like molecule [(2R)-2,4-dihydroxy-4-oxobutyl]-trimethylazanium

Released:
DOI: 10.2210/pdb5n3n/pdb
PDB entry 5n3n coloured by chain, front view.
PDB entry 5n3n coloured by chain, side view.
PDB entry 5n3n coloured by chain, top view.
Source organism: Cricetulus griseus
Entry authors: Siefker C, Heine A, Klebe G

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150424 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
cAMP-dependent protein kinase catalytic subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 351 amino acids
Theoretical weight: 40.92 KDa
Source organism: Cricetulus griseus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P25321 (Residues: 1-351; Coverage: 100%)
Gene name: PRKACA
Sequence domains: Protein kinase domain

Ligands and Environments

3 bound ligands:
Ligand MPD 2 x MPD
Ligand 152 1 x 152
Ligand DMS 1 x DMS
2 modified residues:
Ligand SEP 2 x SEP
Ligand TPO 1 x TPO

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P212121
Unit cell:
a: 51.205Å b: 71.986Å c: 97.665Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.136 0.135 0.153
Expression system: Escherichia coli BL21(DE3)

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