› 5n5s

X-ray diffraction
2.3Å resolution

Crystal structure of aldehyde dehydrogenase 21 (ALDH21) from Physcomitrella patens in complex with NADP+

Released:
DOI: 10.2210/pdb5n5s/pdb
PDB entry 5n5s coloured by chain, front view.
PDB entry 5n5s coloured by chain, side view.
PDB entry 5n5s coloured by chain, top view.
Source organism: Physcomitrium patens
Primary publication:
The ALDH21 gene found in lower plants and some vascular plants codes for a NADP+ -dependent succinic semialdehyde dehydrogenase.
Kopečná M, Vigouroux A, Vilím J, Končitíková R, Briozzo P, Hájková E, Jašková L, von Schwartzenberg K, Šebela M, Moréra S, Kopečný D
Plant J 92 229-243 (2017)
PMID: 28749584

Function and Biology Details

Reaction catalysed: 
D-glyceraldehyde 3-phosphate + NADP(+) + H(2)O = 3-phospho-D-glycerate + NADPH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-501876 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NADP-dependent glyceraldehyde-3-phosphate dehydrogenase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 515 amino acids
Theoretical weight: 56.64 KDa
Source organism: Physcomitrium patens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: A9SS48 (Residues: 1-497; Coverage: 100%)
Gene name: PHYPA_001937
Sequence domains: Aldehyde dehydrogenase family
Structure domains:

Ligands and Environments


Cofactor: Ligand NAP 4 x NAP
1 bound ligand:
Ligand EDO 3 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 2
Spacegroup: P21212
Unit cell:
a: 89.38Å b: 165.28Å c: 138.38Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.183 0.207
Expression system: Escherichia coli BL21

Quick links

Citations

1 review citation

Recent Development on Plant Aldehyde Dehydrogenase Enzymes and Their Functions in Plant Development and Stress Signaling.
Tola et al. (2020)
3 other articles

1 mention without citation

Structural basis for human ZBTB7A action at the fetal globin promoter.
Yang et al. (2021)