› 5n69

X-ray diffraction
2.45Å resolution

Cardiac muscle myosin S1 fragment in the pre-powerstroke state co-crystallized with the activator Omecamtiv Mecarbil

Released:
DOI: 10.2210/pdb5n69/pdb
PDB entry 5n69 coloured by chain, front view.
PDB entry 5n69 coloured by chain, side view.
PDB entry 5n69 coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Mechanistic and structural basis for activation of cardiac myosin force production by omecamtiv mecarbil.
Planelles-Herrero VJ, Hartman JJ, Robert-Paganin J, Malik FI, Houdusse A
OpenAccess logo Nat Commun 8 190 (2017)
PMID: 28775348

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-551375 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Myosin-7 Chains: A, B
Molecule details ›
Chains: A, B
Length: 828 amino acids
Theoretical weight: 94.83 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q9BE39 (Residues: 1-828; Coverage: 43%)
Gene name: MYH7
Sequence domains:
Myosin light chain 3 Chains: G, H
Molecule details ›
Chains: G, H
Length: 199 amino acids
Theoretical weight: 21.97 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P85100 (Residues: 1-199; Coverage: 100%)
Gene name: MYL3
Structure domains: EF-hand

Ligands and Environments

6 bound ligands:
Ligand ADP 2 x ADP
Ligand VO4 2 x VO4
Ligand MG 2 x MG
Ligand 2OW 2 x 2OW
Ligand GOL 25 x GOL
Ligand TCE 1 x TCE
1 modified residue:
Ligand M3L 2 x M3L

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P212121
Unit cell:
a: 98.32Å b: 122.45Å c: 187.43Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.18 0.224

Quick links

Citations

24 review citations

Cardiac myosin super relaxation (SRX): a perspective on fundamental biology, human disease and therapeutics.
Schmid et al. (2021)
23 more

20 mentions without citation

Three perspectives on the molecular basis of hypercontractility caused by hypertrophic cardiomyopathy mutations.
Spudich JA. (2019)
19 more