› 5n7l

X-ray diffraction
2.5Å resolution

Crystal structure of the periplasmic domain of XcpY, tI crystal form.

Released:
DOI: 10.2210/pdb5n7l/pdb
PDB entry 5n7l coloured by chain, front view.
PDB entry 5n7l coloured by chain, side view.
PDB entry 5n7l coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
Structure and oligomerization of the periplasmic domain of GspL from the type II secretion system of Pseudomonas aeruginosa.
Fulara A, Vandenberghe I, Read RJ, Devreese B, Savvides SN
OpenAccess logo Sci Rep 8 16760 (2018)
PMID: 30425318

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150360 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Type II secretion system protein L Chain: A
Molecule details ›
Chain: A
Length: 80 amino acids
Theoretical weight: 8.11 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P25060 (Residues: 303-382; Coverage: 21%)
Gene names: PA3096, xcpY
Sequence domains: GspL periplasmic domain

Ligands and Environments

2 bound ligands:
Ligand BR 3 x BR
Ligand GLU 3 x GLU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 2
Spacegroup: I4122
Unit cell:
a: 100.439Å b: 100.439Å c: 98.848Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.201 0.22
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Architecture, Function, and Substrates of the Type II Secretion System.
Korotkov et al. (2019)
2 more

1 mention without citation

Structure and oligomerization of the periplasmic domain of GspL from the type II secretion system of Pseudomonas aeruginosa.
Fulara et al. (2018)