Function and Biology Details
Reactions catalysed:
SsDNA + n NTP = ssDNA/pppN(pN)(n-1) hybrid + (n-1) diphosphate
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Sequence domains:
Structure analysis Details
Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151051 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
DNA-directed primase/polymerase protein
Molecule details ›
Chain: X
Length: 102 amino acids
Theoretical weight: 11.16 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
Length: 102 amino acids
Theoretical weight: 11.16 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
- Canonical:
Q96LW4 (Residues: 480-560; Coverage: 15%)
Replication protein A 70 kDa DNA-binding subunit, N-terminally processed
Molecule details ›
Chain: A
Length: 121 amino acids
Theoretical weight: 13.35 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
Sequence domains: Replication factor-A protein 1, N-terminal domain
Structure domains: Nucleic acid-binding proteins
Length: 121 amino acids
Theoretical weight: 13.35 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
- Canonical: P27694 (Residues: 1-120; Coverage: 20%)
Sequence domains: Replication factor-A protein 1, N-terminal domain
Structure domains: Nucleic acid-binding proteins
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
wwPDB Validation report is not available for this entry.
X-ray source:
DIAMOND BEAMLINE I04
Spacegroup:
P212121
Expression system: Escherichia coli BL21
