› 5n9d

X-ray diffraction
2.71Å resolution

Crystal Structure of Drosophila DHX36 helicase in complex with GGGTTAGGGT

Released:
DOI: 10.2210/pdb5n9d/pdb
PDB entry 5n9d coloured by chain, front view.
PDB entry 5n9d coloured by chain, side view.
PDB entry 5n9d coloured by chain, top view.
Source organisms:
Primary publication:
Molecular Mechanistic Insights into Drosophila DHX36-Mediated G-Quadruplex Unfolding: A Structure-Based Model.
Chen WF, Rety S, Guo HL, Dai YX, Wu WQ, Liu NN, Auguin D, Liu QW, Hou XM, Dou SX, Xi XG
Structure 26 403-415.e4 (2018)
PMID: 29429875

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-119055 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
RNA helicase Chains: A, B
Molecule details ›
Chains: A, B
Length: 944 amino acids
Theoretical weight: 108.4 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8SWT2 (Residues: 1-942; Coverage: 100%)
Gene names: 38E.15, CG9323, DmDHX36, DmRHAU, Dmel\CG9323, Dmel_CG9323, RHAU, Rhau, cg9323
Sequence domains:
DNA (5'-D(P*GP*GP*GP*TP*TP*AP*GP*GP*GP*T)-3') Chains: C, D
Molecule details ›
Chains: C, D
Length: 10 nucleotides
Theoretical weight: 3.16 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: C2
Unit cell:
a: 305.436Å b: 51.339Å c: 164.947Å
α: 90° β: 114.8° γ: 90°
R-values:
R R work R free
0.188 0.185 0.247
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

8 review citations

The Structure and Function of DNA G-Quadruplexes.
Spiegel et al. (2020)
7 more