› 5naq

X-ray diffraction
2.48Å resolution

Crystal structure of native 6-phospho-glucosidase LpBgl from Lactobacillus plantarum

Released:
DOI: 10.2210/pdb5naq/pdb
PDB entry 5naq coloured by chain, front view.
PDB entry 5naq coloured by chain, side view.
PDB entry 5naq coloured by chain, top view.
Source organism: Lactiplantibacillus plantarum
Primary publication:
Structural basis of the substrate specificity and instability in solution of a glycosidase from Lactobacillus plantarum.
Acebrón I, Plaza-Vinuesa L, de Las Rivas B, Muñoz R, Cumella J, Sánchez-Sancho F, Mancheño JM
Biochim Biophys Acta Proteins Proteom 1865 1227-1236 (2017)
PMID: 28734976

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123765 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-galactosidase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 477 amino acids
Theoretical weight: 54.67 KDa
Source organism: Lactiplantibacillus plantarum
Expression system: Escherichia coli
UniProt:
  • Canonical: F9ULH8 (Residues: 1-461; Coverage: 100%)
Gene names: bgl, lp_3629
Sequence domains: Glycosyl hydrolase family 1
Structure domains: Glycosidases

Ligands and Environments

1 bound ligand:
Ligand PO4 12 x PO4
1 modified residue:
Ligand CAS 6 x CAS

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM16
Spacegroup: C2
Unit cell:
a: 196.688Å b: 191.683Å c: 105.931Å
α: 90° β: 102.75° γ: 90°
R-values:
R R work R free
0.162 0.159 0.217
Expression system: Escherichia coli

Quick links

Citations

2 citation in other articles

Heme-binding enables allosteric modulation in an ancient TIM-barrel glycosidase.
Gamiz-Arco et al. (2021)
1 more