› 5ned

Electron Microscopy
3.1Å resolution

CryoEM Structure of Foot and Mouth Disease Virus O PanAsia

Released:
DOI: 10.2210/pdb5ned/pdb
PDB entry 5ned coloured by chain, front view.
PDB entry 5ned coloured by chain, side view.
PDB entry 5ned coloured by chain, top view.
Source organism: Foot-and-mouth disease virus
Primary publication:
Rules of engagement between αvβ6 integrin and foot-and-mouth disease virus.
Kotecha A, Wang Q, Dong X, Ilca SL, Ondiviela M, Zihe R, Seago J, Charleston B, Fry EE, Abrescia NGA, Springer TA, Huiskonen JT, Stuart DI
Nat Commun 8 15408 (2017)
PMID: 28534487
Related structures: EMD-3630

Function and Biology Details

Reactions catalysed: 
Autocatalytically cleaves itself from the polyprotein of the foot-and-mouth disease virus by hydrolysis of a Lys-|-Gly bond, but then cleaves host cell initiation factor eIF-4G at bonds -Gly-|-Arg- and -Lys-|-Arg-.
NTP + H(2)O = NDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 240-mer (preferred)
PDBe Complex ID:
PDB-CPX-180620 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Viral protein 1 (VP1) Chain: A
Molecule details ›
Chain: A
Length: 211 amino acids
Theoretical weight: 23.34 KDa
Source organism: Foot-and-mouth disease virus
Expression system: Cricetinae gen. sp.
UniProt:
  • Canonical: Q98W00 (Residues: 1-211; Coverage: 99%)
Gene name: 1D
Sequence domains: Picornavirus coat protein
Structure domains: Jelly Rolls
O PanAsia VP2 Chain: B
Molecule details ›
Chain: B
Length: 218 amino acids
Theoretical weight: 24.39 KDa
Source organism: Foot-and-mouth disease virus
Expression system: Cricetinae gen. sp.
UniProt:
  • Canonical: Q6ZZ87 (Residues: 287-504; Coverage: 9%)
Gene name: polyprotein
Sequence domains: picornavirus capsid protein
Structure domains: Jelly Rolls
O PanAsia VP3 Chain: C
Molecule details ›
Chain: C
Length: 220 amino acids
Theoretical weight: 23.94 KDa
Source organism: Foot-and-mouth disease virus
Expression system: Cricetinae gen. sp.
UniProt:
  • Canonical: Q7TD07 (Residues: 505-724; Coverage: 9%)
Sequence domains: picornavirus capsid protein
Structure domains: Jelly Rolls
O PanAsia VP4 Chain: D
Molecule details ›
Chain: D
Length: 85 amino acids
Theoretical weight: 8.79 KDa
Source organism: Foot-and-mouth disease virus
Expression system: Cricetinae gen. sp.
UniProt:
  • Canonical: Q7TD07 (Residues: 202-286; Coverage: 4%)
Sequence domains: Viral protein VP4 subunit
Structure domains: Capsid protein VP4 superfamily, Picornavirus

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.1Å
Relevant EMDB volumes: EMD-3630
Expression system: Cricetinae gen. sp.

Quick links

Citations

15 review citations

Virus-Receptor Interactions: The Key to Cellular Invasion.
Maginnis MS. (2018)
14 more

1 mention without citation

Rules of engagement between αvβ6 integrin and foot-and-mouth disease virus.
Kotecha et al. (2017)