5ngm

Electron Microscopy
2.9Å resolution

2.9S structure of the 70S ribosome composing the S. aureus 100S complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 52-mer (preferred)
PDBe Complex ID:
PDB-CPX-100541 (preferred)
Entry contents:
49 distinct polypeptide molecules
3 distinct RNA molecules
Macromolecules (52 distinct):
Small ribosomal subunit protein uS2 Chain: Ab
Molecule details ›
Chain: Ab
Length: 255 amino acids
Theoretical weight: 29.14 KDa
Source organism: Staphylococcus aureus
UniProt:
  • Canonical: A0A077UDU1 (Residues: 1-255; Coverage: 100%)
Gene names: CD116_13080, ERS140147_00130, NCTC13712_01201, rpsB
Sequence domains: Ribosomal protein S2
Small ribosomal subunit protein uS3 Chain: Ac
Molecule details ›
Chain: Ac
Length: 217 amino acids
Theoretical weight: 24.14 KDa
Source organism: Staphylococcus aureus
UniProt:
  • Canonical: A0A077UU26 (Residues: 1-217; Coverage: 100%)
Gene names: CD116_01830, ERS140147_00632, NCTC13712_02192, rpsC
Sequence domains:
Small ribosomal subunit protein bS6 Chain: Af
Molecule details ›
Chain: Af
Length: 98 amino acids
Theoretical weight: 11.61 KDa
Source organism: Staphylococcus aureus
UniProt:
  • Canonical: W8TPC6 (Residues: 1-98; Coverage: 100%)
Gene names: CD116_05330, ERS140147_02571, NCTC13712_00312, rpsF
Sequence domains: Ribosomal protein S6
Small ribosomal subunit protein uS7 Chain: Ag
Molecule details ›
Chain: Ag
Length: 156 amino acids
Theoretical weight: 17.83 KDa
Source organism: Staphylococcus aureus
UniProt:
  • Canonical: A0A077VHU6 (Residues: 1-156; Coverage: 100%)
Gene names: CD116_12315, ERS140147_01024, NCTC13712_00499, rpsG
Sequence domains: Ribosomal protein S7p/S5e
Small ribosomal subunit protein uS8 Chain: Ah
Molecule details ›
Chain: Ah
Length: 132 amino acids
Theoretical weight: 14.85 KDa
Source organism: Staphylococcus aureus
UniProt:
  • Canonical: W8U8T8 (Residues: 1-132; Coverage: 100%)
Gene names: CD116_01870, ERS140147_00624, NCTC13712_02184, rpsH
Sequence domains: Ribosomal protein S8
Small ribosomal subunit protein uS9 Chain: Ai
Molecule details ›
Chain: Ai
Length: 132 amino acids
Theoretical weight: 14.86 KDa
Source organism: Staphylococcus aureus
UniProt:
  • Canonical: A0A0K7UQP6 (Residues: 1-132; Coverage: 100%)
Gene names: CD116_01965, ERS140147_00604, NCTC13712_02164, rpsI
Sequence domains: Ribosomal protein S9/S16
Small ribosomal subunit protein uS10 Chain: Aj
Molecule details ›
Chain: Aj
Length: 102 amino acids
Theoretical weight: 11.6 KDa
Source organism: Staphylococcus aureus
UniProt:
  • Canonical: A0A0H2K0A0 (Residues: 1-102; Coverage: 100%)
Gene names: CD116_01795, ERS140147_00639, NCTC13712_02199, rpsJ
Sequence domains: Ribosomal protein S10p/S20e
Small ribosomal subunit protein uS11 Chain: Ak
Molecule details ›
Chain: Ak
Length: 129 amino acids
Theoretical weight: 13.91 KDa
Source organism: Staphylococcus aureus
UniProt:
  • Canonical: A0A077UKD6 (Residues: 1-129; Coverage: 100%)
Gene names: CD116_01925, ERS140147_00613, NCTC13712_02173, rpsK
Sequence domains: Ribosomal protein S11
Small ribosomal subunit protein uS12 Chain: Al
Molecule details ›
Chain: Al
Length: 137 amino acids
Theoretical weight: 15.32 KDa
Source organism: Staphylococcus aureus
UniProt:
  • Canonical: W8U1C6 (Residues: 1-137; Coverage: 100%)
Gene names: CD116_12320, ERS140147_01023, NCTC13712_00498, rpsL
Sequence domains: Ribosomal protein S12/S23
Small ribosomal subunit protein uS14 Chain: An
Molecule details ›
Chain: An
Length: 61 amino acids
Theoretical weight: 7.32 KDa
Source organism: Staphylococcus aureus
UniProt:
  • Canonical: A0A077UGW7 (Residues: 1-61; Coverage: 100%)
Gene names: CD116_01865, ERS140147_00625, NCTC13712_02185, rpsN, rpsN1, rpsN_1, rpsZ
Sequence domains: Ribosomal protein S14p/S29e
Small ribosomal subunit protein uS17 Chain: Aq
Molecule details ›
Chain: Aq
Length: 87 amino acids
Theoretical weight: 10.2 KDa
Source organism: Staphylococcus aureus
UniProt:
  • Canonical: A0A077VLD5 (Residues: 1-87; Coverage: 100%)
Gene names: CD116_01845, ERS140147_00629, NCTC13712_02189, rpsQ
Sequence domains: Ribosomal protein S17
Small ribosomal subunit protein bS18 Chain: Ar
Molecule details ›
Chain: Ar
Length: 80 amino acids
Theoretical weight: 9.33 KDa
Source organism: Staphylococcus aureus
UniProt:
  • Canonical: A0A077UZF4 (Residues: 1-80; Coverage: 100%)
Gene names: CD116_05340, ERS140147_02569, NCTC13712_00314, rpsR
Sequence domains: Ribosomal protein S18
Small ribosomal subunit protein uS19 Chain: As
Molecule details ›
Chain: As
Length: 92 amino acids
Theoretical weight: 10.64 KDa
Source organism: Staphylococcus aureus
UniProt:
  • Canonical: A0A077V0P8 (Residues: 1-92; Coverage: 100%)
Gene names: CD116_01820, ERS140147_00634, NCTC13712_02194, rpsS
Sequence domains: Ribosomal protein S19
Small ribosomal subunit protein bS21 Chain: Au
Molecule details ›
Chain: Au
Length: 58 amino acids
Theoretical weight: 6.99 KDa
Source organism: Staphylococcus aureus
UniProt:
  • Canonical: A0A077UHS1 (Residues: 1-58; Coverage: 100%)
Gene names: CD116_08465, ERS140147_00997, NCTC13712_01572, rpsU
Sequence domains: Ribosomal protein S21
Large ribosomal subunit protein uL2 Chain: AC
Molecule details ›
Chain: AC
Length: 277 amino acids
Theoretical weight: 30.22 KDa
Source organism: Staphylococcus aureus
UniProt:
  • Canonical: A0A077W1J0 (Residues: 1-277; Coverage: 100%)
Gene names: CD116_01815, ERS140147_00635, NCTC13712_02195, rplB
Sequence domains:
Large ribosomal subunit protein uL3 Chain: AD
Molecule details ›
Chain: AD
Length: 220 amino acids
Theoretical weight: 23.76 KDa
Source organism: Staphylococcus aureus
UniProt:
  • Canonical: W8U3W0 (Residues: 1-220; Coverage: 100%)
Gene names: CD116_01800, ERS140147_00638, NCTC13712_02198, rplC
Sequence domains: Ribosomal protein L3
Large ribosomal subunit protein uL13 Chain: AH
Molecule details ›
Chain: AH
Length: 145 amino acids
Theoretical weight: 16.36 KDa
Source organism: Staphylococcus aureus
UniProt:
  • Canonical: W8TUE6 (Residues: 1-145; Coverage: 100%)
Gene names: CD116_01960, ERS140147_00605, NCTC13712_02165, rplM
Sequence domains: Ribosomal protein L13
Large ribosomal subunit protein uL14 Chain: AI
Molecule details ›
Chain: AI
Length: 122 amino acids
Theoretical weight: 13.16 KDa
Source organism: Staphylococcus aureus
UniProt:
  • Canonical: A0A077UUA0 (Residues: 1-122; Coverage: 100%)
Gene names: CD116_01850, ERS140147_00628, NCTC13712_02188, rplN
Sequence domains: Ribosomal protein L14p/L23e
Large ribosomal subunit protein uL15 Chain: AJ
Molecule details ›
Chain: AJ
Length: 146 amino acids
Theoretical weight: 15.63 KDa
Source organism: Staphylococcus aureus
UniProt:
  • Canonical: A0A077UGA7 (Residues: 1-146; Coverage: 100%)
Gene names: CD116_01895, ERS140147_00619, NCTC13712_02179, rplO
Sequence domains: Ribosomal proteins 50S-L15, 50S-L18e, 60S-L27A
Large ribosomal subunit protein uL16 Chain: AK
Molecule details ›
Chain: AK
Length: 144 amino acids
Theoretical weight: 16.27 KDa
Source organism: Staphylococcus aureus
UniProt:
  • Canonical: A0A077V4G0 (Residues: 1-144; Coverage: 100%)
Gene names: CD116_01835, ERS140147_00631, NCTC13712_02191, rplP
Sequence domains: Ribosomal protein L16p/L10e
Large ribosomal subunit protein bL17 Chain: AL
Molecule details ›
Chain: AL
Length: 122 amino acids
Theoretical weight: 13.77 KDa
Source organism: Staphylococcus aureus
UniProt:
  • Canonical: A0A077UG91 (Residues: 1-122; Coverage: 100%)
Gene names: CD116_01935, ERS140147_00611, NCTC13712_02171, rplQ
Sequence domains: Ribosomal protein L17
Large ribosomal subunit protein bL19 Chain: AN
Molecule details ›
Chain: AN
Length: 116 amino acids
Theoretical weight: 13.39 KDa
Source organism: Staphylococcus aureus
UniProt:
  • Canonical: A0A077UVB6 (Residues: 1-116; Coverage: 100%)
Gene names: CD116_13000, ERS140147_00145, NCTC13712_01186, rplS
Sequence domains: Ribosomal protein L19
Large ribosomal subunit protein bL20 Chain: AO
Molecule details ›
Chain: AO
Length: 118 amino acids
Theoretical weight: 13.72 KDa
Source organism: Staphylococcus aureus
UniProt:
  • Canonical: A0A077VMP6 (Residues: 1-118; Coverage: 100%)
Gene names: CD116_08935, ERS140147_00901, NCTC13712_01667, rplT
Sequence domains: Ribosomal protein L20
Large ribosomal subunit protein uL22 Chain: AQ
Molecule details ›
Chain: AQ
Length: 117 amino acids
Theoretical weight: 12.86 KDa
Source organism: Staphylococcus aureus
UniProt:
  • Canonical: A0A077UKF9 (Residues: 1-117; Coverage: 100%)
Gene names: CD116_01825, ERS140147_00633, NCTC13712_02193, rplV
Sequence domains: Ribosomal protein L22p/L17e
Large ribosomal subunit protein bL25 Chain: AT
Molecule details ›
Chain: AT
Length: 217 amino acids
Theoretical weight: 23.81 KDa
Source organism: Staphylococcus aureus
UniProt:
  • Canonical: A0A133Q8Z9 (Residues: 3-219; Coverage: 99%)
Gene names: HMPREF3211_00359, ctc, rplY
Sequence domains:
50S ribosomal protein L27 Chain: AU
Molecule details ›
Chain: AU
Length: 94 amino acids
Theoretical weight: 10.33 KDa
Source organism: Staphylococcus aureus
Large ribosomal subunit protein bL28 Chain: AV
Molecule details ›
Chain: AV
Length: 62 amino acids
Theoretical weight: 7 KDa
Source organism: Staphylococcus aureus
UniProt:
  • Canonical: A0A077URJ8 (Residues: 1-62; Coverage: 100%)
Gene names: CD116_12910, ERS140147_00162, NCTC13712_01169, rpmB
Sequence domains: Ribosomal L28 family
Large ribosomal subunit protein uL29 Chain: AW
Molecule details ›
Chain: AW
Length: 73 amino acids
Theoretical weight: 8.59 KDa
Source organism: Staphylococcus aureus
UniProt:
  • Canonical: A0A0H3KED7 (Residues: 1-73; Coverage: 100%)
Gene names: NWMN_2144, rpmC
Sequence domains: Ribosomal L29 protein
Large ribosomal subunit protein bL32 Chain: AZ
Molecule details ›
Chain: AZ
Length: 57 amino acids
Theoretical weight: 6.5 KDa
Source organism: Staphylococcus aureus
UniProt:
  • Canonical: A0A077UWR7 (Residues: 1-57; Coverage: 100%)
Gene names: CD116_02980, ERS140147_01255, NCTC13712_01072, rpmF
Sequence domains: Ribosomal L32p protein family
Large ribosomal subunit protein bL33 Chain: A1
Molecule details ›
Chain: A1
Length: 49 amino acids
Theoretical weight: 5.94 KDa
Source organism: Staphylococcus aureus
UniProt:
  • Canonical: A0A077V2P0 (Residues: 1-49; Coverage: 100%)
Gene names: CD116_07290, ERS140147_01724, NCTC13712_01328, rpmG, rpmG2, rpmG2_1
Sequence domains: Ribosomal protein L33
Large ribosomal subunit protein bL34 Chain: A2
Molecule details ›
Chain: A2
Length: 45 amino acids
Theoretical weight: 5.45 KDa
Source organism: Staphylococcus aureus
UniProt:
  • Canonical: Q2YZB6 (Residues: 1-45; Coverage: 100%)
Gene names: SAB2590c, rpmH
Sequence domains: Ribosomal protein L34
Large ribosomal subunit protein bL35 Chain: A3
Molecule details ›
Chain: A3
Length: 66 amino acids
Theoretical weight: 7.72 KDa
Source organism: Staphylococcus aureus
UniProt:
  • Canonical: A0A077UL47 (Residues: 1-66; Coverage: 100%)
Gene names: CD116_08940, ERS140147_00900, NCTC13712_01668, rpmI
Sequence domains: Ribosomal protein L35
Large ribosomal subunit protein bL36 Chain: A4
Molecule details ›
Chain: A4
Length: 37 amino acids
Theoretical weight: 4.32 KDa
Source organism: Staphylococcus aureus
UniProt:
  • Canonical: A0A077UGV8 (Residues: 1-37; Coverage: 100%)
Gene names: CD116_01915, ERS140147_00615, NCTC13712_02175, rpmJ
Sequence domains: Ribosomal protein L36
16S ribosomal RNA Chain: Aa
Molecule details ›
Chain: Aa
Length: 1555 nucleotides
Theoretical weight: 502.98 KDa
Sequence domains: Bacterial small subunit ribosomal RNA
23S ribosomal RNA Chain: AA
Molecule details ›
Chain: AA
Length: 2923 nucleotides
Theoretical weight: 946.7 KDa
Sequence domains: Bacterial large subunit ribosomal RNA
5S ribosomal RNA Chain: AB
Molecule details ›
Chain: AB
Length: 115 nucleotides
Theoretical weight: 36.97 KDa
Sequence domains: 5S ribosomal RNA

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.9Å
Relevant EMDB volumes: EMD-3640