› 5nry

X-ray diffraction
1.85Å resolution

Cys-Gly dipeptidase GliJ in complex with Fe3+

Released:
DOI: 10.2210/pdb5nry/pdb
PDB entry 5nry coloured by chain, front view.
PDB entry 5nry coloured by chain, side view.
PDB entry 5nry coloured by chain, top view.
Source organism: Aspergillus fumigatus Af293
Primary publication:
Gliotoxin Biosynthesis: Structure, Mechanism, and Metal Promiscuity of Carboxypeptidase GliJ.
Marion A, Groll M, Scharf DH, Scherlach K, Glaser M, Sievers H, Schuster M, Hertweck C, Brakhage AA, Antes I, Huber EM
ACS Chem Biol 12 1874-1882 (2017)
PMID: 28525266

Function and Biology Details

Reaction catalysed: 
Hydrolysis of dipeptides.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175833 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dipeptidase gliJ Chain: A
Molecule details ›
Chain: A
Length: 401 amino acids
Theoretical weight: 45.01 KDa
Source organism: Aspergillus fumigatus Af293
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q4WMJ8 (Residues: 1-388; Coverage: 100%)
Gene names: AFUA_6G09650, gliJ
Sequence domains: Membrane dipeptidase (Peptidase family M19)
Structure domains: Metal-dependent hydrolases

Ligands and Environments

2 bound ligands:
Ligand FE 2 x FE
Ligand CL 3 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P3221
Unit cell:
a: 98.99Å b: 98.99Å c: 106.3Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.166 0.165 0.19
Expression system: Escherichia coli BL21(DE3)

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Citations

4 review citations

Enzymes for Detoxification of Various Mycotoxins: Origins and Mechanisms of Catalytic Action.
Lyagin et al. (2019)
3 more