Function and Biology Details
Reaction catalysed:
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Structure analysis Details
Assembly composition:
hetero dimer (preferred)
Assembly name:
Tyrosine-protein kinase HCK and Protein Nef (preferred)
PDBe Complex ID:
PDB-CPX-140250 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protein Nef
Molecule details ›
Chains: A, B
Length: 172 amino acids
Theoretical weight: 20.22 KDa
Source organism: Simian immunodeficiency virus
Expression system: Escherichia coli
UniProt:
Sequence domains: Negative factor, (F-Protein) or Nef
Structure domains: Nef Regulatory Factor
Length: 172 amino acids
Theoretical weight: 20.22 KDa
Source organism: Simian immunodeficiency virus
Expression system: Escherichia coli
UniProt:
- Canonical:
Q5QGG3 (Residues: 68-235; Coverage: 64%)
Sequence domains: Negative factor, (F-Protein) or Nef
Structure domains: Nef Regulatory Factor
Tyrosine-protein kinase HCK
Molecule details ›
Chains: C, D
Length: 60 amino acids
Theoretical weight: 6.99 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
Sequence domains: SH3 domain
Structure domains: SH3 Domains
Length: 60 amino acids
Theoretical weight: 6.99 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
- Canonical: P08631 (Residues: 79-90, 96-138; Coverage: 11%)
Sequence domains: SH3 domain
Structure domains: SH3 Domains
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
SLS BEAMLINE X06SA
Spacegroup:
P32
Expression system: Escherichia coli
