› 5nun

X-ray diffraction
1.95Å resolution

Engineered beta-lactoglobulin: variant F105L-L39A-M107F in complex with chlorpromazine (LG-LAF-CLP)

Released:
DOI: 10.2210/pdb5nun/pdb
PDB entry 5nun coloured by chain, front view.
PDB entry 5nun coloured by chain, side view.
PDB entry 5nun coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
The engineered β-lactoglobulin with complementarity to the chlorpromazine chiral conformers.
Loch JI, Bonarek P, Tworzydło M, Łazińska I, Szydłowska J, Lipowska J, Rzęsikowska K, Lewiński K
Int J Biol Macromol 114 85-96 (2018)
PMID: 29555509

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136154 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-lactoglobulin Chain: A
Molecule details ›
Chain: A
Length: 162 amino acids
Theoretical weight: 18.17 KDa
Source organism: Bos taurus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P02754 (Residues: 17-178; Coverage: 100%)
Gene name: LGB
Sequence domains: Lipocalin / cytosolic fatty-acid binding protein family
Structure domains: Lipocalin

Ligands and Environments

1 bound ligand:
Ligand Z80 1 x Z80
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: OXFORD DIFFRACTION NOVA
Spacegroup: P3221
Unit cell:
a: 53.657Å b: 53.657Å c: 110.7Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.219 0.215 0.262
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

β-Lactoglobulin and Glycodelin: Two Sides of the Same Coin?
Sawyer L. (2021)
2 other articles

1 mention without citation

Conjugates of Methylene Blue with Cycloalkaneindoles as New Multifunctional Agents for Potential Treatment of Neurodegenerative Disease.
Bachurin et al. (2022)