› 5nyb

X-ray diffraction
1.88Å resolution

A C145A mutant of Nesterenkonia AN1 amidase bound to adipamide

Released:
DOI: 10.2210/pdb5nyb/pdb
PDB entry 5nyb coloured by chain, front view.
PDB entry 5nyb coloured by chain, side view.
PDB entry 5nyb coloured by chain, top view.
Source organism: Nesterenkonia sp. 10004
Entry authors: Kimani SW, Sewell BT

Function and Biology Details

Reaction catalysed: 
A monocarboxylic acid amide + H(2)O = a monocarboxylate + NH(3)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112174 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CN hydrolase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 283 amino acids
Theoretical weight: 30.07 KDa
Source organism: Nesterenkonia sp. 10004
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: D0VWZ1 (Residues: 1-263; Coverage: 100%)
Gene name: Nit2
Sequence domains: Carbon-nitrogen hydrolase

Ligands and Environments

1 bound ligand:
Ligand 9EE 1 x 9EE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: C2221
Unit cell:
a: 76.254Å b: 114.769Å c: 64.842Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 0.168 0.215
Expression system: Escherichia coli BL21(DE3)

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